Histopaque®-1119
SIGMA/11191 - sterile-filtered, density: 1.119
Product Type: Chemical
Histopaque®-1119 is suitable for use in hematological and histological applications. Photo is meant to be representative of packaging you will receive if ordered. Appropriate product information is printed on actual labels and package types are subject to change.
Suitable for use in hematological and histological applications. Photo is meant to be representative of packaging you will receive if ordered. Appropriate product information is printed on actual labels and package types are subject to change.
| application(s) | hematology histology |
| density | 1.119 |
| form | liquid |
| impurities | endotoxin, tested |
| Quality Level | 400  |
| shelf life | Expiry date on the label. |
| sterility | sterile-filtered |
| storage temp. | 2-8°C |
| technique(s) | cell culture | mammalian: suitable |
| Application: | Histopaque®-1119 is suitable for use in conjunction with Histopaque-1077 as it facilitates the rapid recovery of viable mononuclear cells and granulocytes from small volumes of whole blood or bone marrow |
| Biochem/physiol Actions: | The process involves the formation of a double gradient on layering an equal volume of Histopaque-1077 over Histopaque-1119. Anticoagulated whole blood is carefully layered onto the upper Histopaque-1077 medium. Upon centrifugation, the erythrocytes are aggregated by polysucrose and thus rapidly sediment. Granulocytes are found at the lower Histopaque-1077/1119 interface; whereas, lymphocytes and other mononuclear cells are found at the upper plasma/Histopaque-1077 interface. Most extraneous platelets found in the upper interface are removed by low-speed centrifugation during washing steps. Erythrocyte contamination is negligible. |
| Features and Benefits: | Histopaque®-1119 offers numerous advantages, such as:• Optimal recovery of viable cells • Permits selective separation of blood cell types • Prevents cell distortion • Preserves cell viability • Minimal extraneous cell interference • Reproducible performance from batch to batch • Sterile-filtered and endotoxin-tested • Stable at 2-8°C for at least 2 years when protected from light |
| General description: | Histopaque®-1119 is a sterile, endotoxin tested, ready-to-use medium. This solution consists of polysucrose and sodium diatrizoate, adjusted to a relative density of 1.119 g/mL and pH 8.8–9.0. |
| Legal Information: | Histopaque is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Symbol |  GHS05 |
| Signal word | Warning |
| Hazard statements | H290 - H315 - H319 |
| Precautionary statements | P234 - P264 - P280 - P302 + P352 - P305 + P351 + P338 - P332 + P313 |
| Hazard Codes | Xi |
| Risk Statements | 36/38 |
| Safety Statements | 26 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Density | 1.119 |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352207 |