CD16 (SP175) Rabbit Monoclonal Antibody
SIGMA/116R-1
Product Type: Chemical
Immunohistochemistry CD16 (SP175) on acute myeloid leukemia
| antibody form | culture supernatant |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | SP175, monoclonal |
| conjugate | unconjugated |
| control | tonsil |
| description | For In Vitro Diagnostic Use in Select Regions (See Chart) |
| form | buffered aqueous solution |
| isotype | IgG |
| manufacturer/tradename | Cell Marque™ |
| packaging | bottle of 1.0 mL predilute (116R-17) |
| bottle of 7.0 mL predilute (116R-18) | |
| vial of 0.1 mL concentrate (116R-14) | |
| vial of 0.5 mL concentrate (116R-15) | |
| vial of 1.0 mL concentrate (116R-16) | |
| Quality Level | 100  |
| 500 |
|
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | 2-8°C |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:25-1:100 |
| visualization | cytoplasmic, membranous |
| General description: | CD16 is a biomarker associated with monocytes and natural killer (NK) cells of the lymphoid lineage. Anti-CD16 immunohistochemistry is useful in differentially diagnosing hepatosplenic gamma delta T-cell lymphoma and gamma delta T-cell large granular lymphocyte leukemia from other peripheral T-cell lymphomas, such as mucosal and cutaneous gamma delta T-cell lymphoma. It is reported that 58% of hepatosplenic gamma delta T-cell lymphomas express CD16, and 86% of gamma delta T-cell large granular lymphocyte leukemias are immunoreactive with anti-CD16. Mucosal and cutaneous gamma delta T-cell lymphomas usually do not express CD16 antigen. A significant decrease can be seen in the number of granulocytes expressing CD16 in chronic myelomonocytic leukemia compared to chronic myelogenous leukemia and control bone marrow biopsy, probably related to dysgranulopoiesis. Bone marrow biopsy immunohistochemistry can be helpful in CMML by identifying both the monocyte expansion and the dysgranulopoiesis with anti-CD16. | ||||
| General description: | CD16 is a receptor on monocytes and natural killer (NK) cells, which facilitates antibody-dependent cellular cytotoxicity. CD16 is a useful marker in NK neoplasms and in certain T-cell neoplasms such as T-cell large granular lymphocytic leukemia. | ||||
| Legal Information: | Cell Marque is a trademark of Merck KGaA, Darmstadt, Germany | ||||
| Linkage: | CD16 Positive Control Slides, Product No. 116S , are available for immunohistochemistry (formalin-fixed, paraffin-embedded sections). |
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| Other Notes: | For Technical Service please contact: 800-665-7284 or email: service@cellmarque.com | ||||
| Physical form: | Solution in Tris Buffer, pH 7.3-7.7, with 1% BSA and <0.1% Sodium Azide | ||||
| Preparation Note: | Download the IFU specific to your product lot and format  Note: This requires a keycode which can be found on your packaging or product label. |
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| Quality: |
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| RIDADR | NONH for all modes of transport |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |