Fetal Bovine Serum

SIGMA/12103C - USA origin, suitable for cell culture

Synonym: Fetal Calf Sera; Fetal Calf Serum; FBS; FCS; sera; serum

MDL Number: MFCD00132239
Product Type: Chemical

Catalog Number	PKG Qty.	Price	Quantity


45-12103C-100ML	100 mL	$338.00 1/EAAdd To Favorites		Add To Favorites
45-12103C-500ML	500 mL	$1150.00 1/EAAdd To Favorites		Add To Favorites
45-12103C-1000ML	1000 mL	DISCONTINUED

biological source	bovine fetus
composition	hemoglobin, ≤20 mg/dL
impurities	≤10 EU/mL endotoxin
origin	USA origin
product line	FBS Premier
Quality Level	500
shipped in	dry ice
storage temp.	−20°C
technique(s)	cell culture \| mammalian: suitable

Analysis Note:	• Endotoxin and hemoglobin tested
Analysis Note:	• Tested for the presence of bacteria, virus, and mycoplasma • Triple filtered with 0.1 micron membrane under aseptic conditions
Application:	Fetal Bovine Serum was used in different types of cell culture as a supplement, such as the following: • PC12 cells grown in Dulbecco′s modified Eagle′s medium supplemented with Fetal Bovine Serum along with other components. • HeLa cells were maintained in Roswell Park Memorial Institute (RPMI) 1640 medium containing Fetal Bovine Serum along with other components. • BaF3 Cell Culture (cells were maintained in RPMI 1640 medium supplemented with Fetal Bovine Serum along with other components). • Primary hippocampal cell cultures (cells were maintained in Eagle′s minimum essential medium and supplemented with heat-inactivated fetal bovine serum along with other components). • HeLa, Cos1 and Cos7 cells were cultured in Dulbecco modified Eagle medium (DMEM) with fetal bovine serum along with other components. Fetal Bovine Serum (FBS) is the most common serum used to supplement cell culture media due to its high nutritional content. Although it is relatively low in protein, FBS is effective in promoting and sustaining growth of vertebrate mammalian and insect cells. This product is for further manufacturing use. THIS PRODUCT IS NOT INTENDED FOR HUMAN OR THERAPEUTIC USE.
General description:	FBS remains a popular media supplement because it provides a wide array of functions in cell culture. FBS delivers nutrients, growth and attachment factors and protects cells from oxidative damage and apoptosis by mechanisms that are difficult to reproduce in serum-free media (SFM) systems.