CD25 (4C9) Mouse Monoclonal Antibody
SIGMA/125M-1
Product Type: Chemical
Immunohistochemistry CD25 (4C9) on adult T-cell lymphoma
| antibody form | culture supernatant |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 4C9, monoclonal |
| conjugate | unconjugated |
| control | lesions of mastocytosis |
| description | For In Vitro Diagnostic Use in Select Regions (See Chart) |
| form | buffered aqueous solution |
| isotype | IgG2b |
| manufacturer/tradename | Cell Marque™ |
| packaging | bottle of 1.0 mL predilute (125M-17) |
| bottle of 7.0 mL predilute (125M-18) | |
| vial of 0.1 mL concentrate (125M-14) | |
| vial of 0.5 mL concentrate (125M-15) | |
| vial of 1.0 mL concentrate (125M-16) | |
| Quality Level | 100  |
| 500 |
|
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | 2-8°C |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:10-1:50 |
| visualization | cytoplasmic, membranous |
| General description: | According to the World Health Organization classification system, the major diagnostic criterion for bone marrow involvement by Systemic Mastocytosis (SM) is the presence of dense aggregates (>15 cells) of mast cells. Expression of CD25, a low-affinity receptor for interleukin-2 (IL-2), is a reliable diagnostic tool for distinguishing neoplastic mast cell aggregates from reactive proliferations, and has therefore recently become a minor criterion for the diagnosis of SM. Hahn et al. demonstrated that aberrant staining of Mast cell clusters by anti-CD25 antibody in GI biopsies was essentially diagnostic of SM. Anti-CD25 antibody has also been useful in identifying mast cells in skin biopsies in the setting of Urticaria Pigmentosa, which is predictive of Systemic Mastocytosis. Quantitation of regulatory T cells (Treg) in the setting of hepatocellular carcinoma has been used as an independent predictive factor of tumor recurrence after hepatic resection for HCC. Also, the percentage of tumor-infiltrating CD25+FOXP3+ regulatory T cells among tumor cells, inside tumor parenchyma and at its periphery, is significantly higher in recurrent cutaneous melanoma than in non-recurrent melanoma. | ||||
| Legal Information: | Cell Marque is a trademark of Merck KGaA, Darmstadt, Germany | ||||
| Linkage: | CD25 Positive Control Slides, Product No. 125S , are available for immunohistochemistry (formalin-fixed, paraffin-embedded sections). |
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| Other Notes: | For Technical Service please contact: 800-665-7284 or email: service@cellmarque.com | ||||
| Physical form: | Solution in Tris Buffer, pH 7.3-7.7, with 1% BSA and <0.1% Sodium Azide | ||||
| Preparation Note: | Download the IFU specific to your product lot and format  Note: This requires a keycode which can be found on your packaging or product label. |
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| Quality: |
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| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352200 |