CD25 (4C9) Mouse Monoclonal Antibody

SIGMA/125M-1

Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-125M-14	0.1 mL	$0.00 1/EA CALL FOR PRICEAdd To Favorites	Add To Favorites
45-125M-15	0.5 mL	$0.00 1/EA CALL FOR PRICEAdd To Favorites	Add To Favorites
45-125M-16	1 mL	$0.00 1/EA CALL FOR PRICEAdd To Favorites	Add To Favorites
45-125M-17	1 mL	$0.00 1/EA CALL FOR PRICEAdd To Favorites	Add To Favorites
45-125M-18	7 mL	$0.00 1/EA CALL FOR PRICEAdd To Favorites	Add To Favorites

Immunohistochemistry CD25 (4C9) on adult T-cell lymphoma

antibody form	culture supernatant
antibody product type	primary antibodies
biological source	mouse
clone	4C9, monoclonal
conjugate	unconjugated
control	lesions of mastocytosis
description	For In Vitro Diagnostic Use in Select Regions (See Chart)
form	buffered aqueous solution
isotype	IgG2b
manufacturer/tradename	Cell Marque^™
packaging	bottle of 1.0 mL predilute (125M-17)
	bottle of 7.0 mL predilute (125M-18)
	vial of 0.1 mL concentrate (125M-14)
	vial of 0.5 mL concentrate (125M-15)
	vial of 1.0 mL concentrate (125M-16)
Quality Level	100
	500
shipped in	wet ice
species reactivity	human
storage temp.	2-8°C
technique(s)	immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:10-1:50
visualization	cytoplasmic, membranous

General description:

According to the World Health Organization classification system, the major diagnostic criterion for bone marrow involvement by Systemic Mastocytosis (SM) is the presence of dense aggregates (>15 cells) of mast cells. Expression of CD25, a low-affinity receptor for interleukin-2 (IL-2), is a reliable diagnostic tool for distinguishing neoplastic mast cell aggregates from reactive proliferations, and has therefore recently become a minor criterion for the diagnosis of SM. Hahn et al. demonstrated that aberrant staining of Mast cell clusters by anti-CD25 antibody in GI biopsies was essentially diagnostic of SM. Anti-CD25 antibody has also been useful in identifying mast cells in skin biopsies in the setting of Urticaria Pigmentosa, which is predictive of Systemic Mastocytosis. Quantitation of regulatory T cells (Treg) in the setting of hepatocellular carcinoma has been used as an independent predictive factor of tumor recurrence after hepatic resection for HCC. Also, the percentage of tumor-infiltrating CD25+FOXP3+ regulatory T cells among tumor cells, inside tumor parenchyma and at its periphery, is significantly higher in recurrent cutaneous melanoma than in non-recurrent melanoma.

Legal Information:

Cell Marque is a trademark of Merck KGaA, Darmstadt, Germany

Linkage:

CD25 Positive Control Slides, Product No. 125S External link

, are available for immunohistochemistry (formalin-fixed, paraffin-embedded sections).

Other Notes:

For Technical Service please contact: 800-665-7284 or email: service@cellmarque.com

Physical form:

Solution in Tris Buffer, pH 7.3-7.7, with 1% BSA and <0.1% Sodium Azide

Preparation Note:

Download the IFU specific to your product lot and format External link

Note: This requires a keycode which can be found on your packaging or product label.

Quality:

IVD

RUO

RIDADR	NONH for all modes of transport
WGK Germany	WGK 2
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Storage Temp.	2-8°C
UNSPSC	12352203