CHO-K1-AC-free

SIGMA/13080801

Product Type: Chemical

Catalog Number	PKG Qty.	Price	Quantity


45-13080801-1VL	1 vial	$0.00 1/EA CALL FOR PRICEAdd To Favorites		Add To Favorites

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biological source	hamster ovary
description	Hamster Chinese Ovary, serum-free
growth mode	suspension
morphology	Aggregates in suspension
Quality Level	100
shipped in	dry ice
storage temp.	−196°C
technique(s)	cell culture \| mammalian: suitable

Cell Line Description:	This is a derviative of the CHO-K1 cell line, ECACC catalogue no. 85051005 which has been adapted to grow in the animal component free medium, CHO PF Media (C5467)
Culture Medium:	CHO PF Media (C5467) + 8mM L-Glutamine (G7513) . When freezing cells use freeze medium composed of 50:50 fresh:conditioned medium + 10% DMSO.
Other Notes:	Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Other Notes:	Cultures from PHE Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
Subculture Routine:	Maintain cultures between approximately 3-9 x 10⁵ cells/ml; 5% CO₂; 37°C. Cells grow as aggregates in suspension. Viability may be poor on resuscitation and may initially decrease further. Full recovery may take up to 2 weeks. A centrifugation step to remove the cryoprotectant is essential. Rapidly thaw the frozen ampoule in a water bath at 37°C for 1-2 minutes. Transfer the contents to a centrifuge tube and slowly add 5-10ml of pre-warmed growth media. Remove a sample for counting. Centrifuge at 100g for 2-3 minutes to pellet cells and seed at a relatively high density of 5-7 x 10⁵ cells/ml. Leave culture flask upright and observe regularly until viable proliferating cells are seen. Once established use a split ratio of 1:2 approximately every 4 to 5 days.