EX-CELL^® 302 Serum-Free Medium for CHO Cells

SIGMA/14324C - without L-glutamine, liquid, sterile-filtered, suitable for cell culture

MDL Number: MFCD01634638
Product Type: Chemical

Catalog Number	PKG Qty.	Price	Quantity


45-14324C-500ML	500 mL	$68.00 1/EAAdd To Favorites		Add To Favorites
45-14324C-1000ML	1000 mL	$118.00 1/EAAdd To Favorites		Add To Favorites

components	L-glutamine: no NaHCO₃: 1.60 g/L HEPES: 7.5 mM glucose: 3.42 g/L phenol red: yes
description	for research or for further manufacturing use
form	liquid
Quality Level	500
shipped in	ambient
sterility	sterile-filtered
storage temp.	2-8°C
technique(s)	cell culture \| mammalian: suitable

Application:	EX-CELL 302 is a serum-free medium which has been specifically developed for the long-term growth of transformed Chinese Hamster Ovary (CHO) cells in suspension for the expression of antibodies or protein products. EX-CELL 302 is an appropriate medium for use with the DHFR^-or Glutamine Synthetase, or the GS System^™, because it does not contain hypoxanthine, thymidine or L-glutamine. For use with the DHFR^- selection system we recommend the addition of 4 mM L-glutamine for optimal growth. Due to current regulatory concerns about the sources of raw materials, EX-CELL 302 was developed using only recombinant human proteins that have molecular weights less than 10 kDa. The total protein concentration found in EX-CELL 302 is less than 1 mg/L. Lutrol has been added at a final concentration of 0.1% to EX-CELL 302 to protect against shear damage in sparged bioreactor systems.
Caution:	Do not freeze. Protect from light. Several components of the medium are light labile and should not be exposed to light for lengthy periods of time.
Legal Information:	EX-CELL is a registered trademark of Merck KGaA, Darmstadt, Germany
Legal Information:	GS System is a trademark of Lonza Group Ltd.