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EX-CELL® 302 Serum-Free Medium for CHO Cells

SIGMA/14324C - without L-glutamine, liquid, sterile-filtered, suitable for cell culture

MDL Number: MFCD01634638
Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-14324C-500ML 500 mL
 $71.00
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45-14324C-1000ML 1000 mL
 $123.00
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components L-glutamine: no
NaHCO3: 1.60 g/L
HEPES: 7.5 mM
glucose: 3.42 g/L
phenol red: yes
description for research or for further manufacturing use
form liquid
Quality Level 500 
shipped in ambient
sterility sterile-filtered
storage temp. 2-8°C
technique(s) cell culture | mammalian: suitable
Application: EX-CELL 302 is a serum-free medium which has been specifically developed for the long-term growth of transformed Chinese Hamster Ovary (CHO) cells in suspension for the expression of antibodies or protein products. EX-CELL 302 is an appropriate medium for use with the DHFR- or Glutamine Synthetase, or the GS System™, because it does not contain hypoxanthine, thymidine or L-glutamine. For use with the DHFR- selection system we recommend the addition of 4 mM L-glutamine for optimal growth. Due to current regulatory concerns about the sources of raw materials, EX-CELL 302 was developed using only recombinant human proteins that have molecular weights less than 10 kDa. The total protein concentration found in EX-CELL 302 is less than 1 mg/L. Lutrol has been added at a final concentration of 0.1% to EX-CELL 302 to protect against shear damage in sparged bioreactor systems.
Caution: Do not freeze. Protect from light. Several components of the medium are light labile and should not be exposed to light for lengthy periods of time.
Legal Information: EX-CELL is a registered trademark of Merck KGaA, Darmstadt, Germany
Legal Information: GS System is a trademark of Lonza Group Ltd.
RIDADR NONH for all modes of transport
WGK Germany WGK 1
Flash Point(F) Not applicable
Flash Point(C) Not applicable
Storage Temp. 2-8°C
UNSPSC 12352207

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