EX-CELL® CD HEK293 Viral Vector Medium
SIGMA/14385C - Chemically defined, animal component-free, without L-glutamine, liquid, sterile-filtered, suitable for cell culture
Synonym: HEK293/HEK293T medium for viral vector production
Product Type: Chemical
Photo is meant to be representative of packaging you will receive if ordered. Appropriate product information is printed on actual labels and package types are subject to change.
| form | liquid |
| packaging | bag of 10 L |
| bottle of 1000 mL | |
| Quality Level | 500  |
| shipped in | wet ice |
| sterility | sterile-filtered |
| storage condition | protect from light |
| storage temp. | 2-8°C |
| technique(s) | cell culture | mammalian: suitable |
| Application: | EX-CELL CD HEK293 Viral Vector Medium was developed to support VirusExpress Production Platforms. It performs well within the platform as well as with other HEK293 clones. The following white paper demonstrates the use of one of the prototype media formulations in developing a scalable, transient transfection-based suspension process for lentiviral vector production using the VirusExpress 293T Lentiviral Production cells: VirusExpress White Paper  |
| General description: | EX-CELL CD HEK293 Viral Vector Medium is an animal origin-free, chemically defined cell culture medium for clinical and commercial manufacturing of viral vectors for gene and cell therapies. The medium is intended for processes implementing (polyethylenimine) PEI transfection reagents. EX-CELL CD HEK293 Viral Vector Medium is part of the VirusExpress® Lentiviral Production Platform and the medium of choice for VirusExpress 293T Lentiviral Production Cells (VP001). The medium is also suitable as a standalone solution for other HEK293 and HEK293T cell lines for AAV and lentiviral manufacturing. EX-CELL CD HEK293 Viral Vector Medium has been formulated to support high suspension cell densities with minimal clumping, high transient transfection efficiency when used with PEI, and high viral titers in shake flask and stirred tank bioreactor formats. Viral vector production using this medium is a scalable, suspension process that does not require a media exchange or feed post-transfection. EX-CELL CD HEK293 Viral Vector Medium has been designed with long-term needs for manufacturing in mind, with a sustainable raw material supply chain, custom packaging options, and careful material and manufacturing process selection. The product is produced in an ISO9001-certified facility following the principles of cGMP according to IPEC-PQG. |
| Legal Information: | EX-CELL is a registered trademark of Sigma-Aldrich Co. LLC |
| Legal Information: | VirusExpress is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Preparation Note: | EX-CELL CD HEK293 Viral Vector Medium is formulated without L-glutamine. Add L-glutamine (Catalog number 59202C, 200 mM) to 6 mM final concentration using aseptic supplementation technique. |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |