EX-CELL^® 420 Serum-Free Medium for Insect Cells

SIGMA/14420C - with L-glutamine, sterile-filtered, suitable for insect cell culture

MDL Number: MFCD01634638
Product Type: Chemical

Catalog Number	PKG Qty.	Price	Quantity


45-14420C-500ML	500 mL	$74.00 1/EAAdd To Favorites		Add To Favorites
45-14420C-1000ML	1000 mL	$135.00 1/EAAdd To Favorites		Add To Favorites

components	HEPES: no L-glutamine: yes glucose: yes NaHCO₃: yes
description	for research or for further manufacturing use
form	liquid
product line	EX-CELL^®
Quality Level	500
shipped in	ambient
sterility	sterile-filtered
storage temp.	2-8°C
technique(s)	cell culture \| insect: suitable

Application:	EX-CELL 420 is a complete medium developed and optimized for the serum-free growth of Sf9 and Sf21 insect cell lines. Cells can be subcultured directly into EX-CELL 420 from serum-free or serum-supplemented media without adaptation. Cultures in EX-CELL 420 routinely reach cell densities greater than 1 × 10⁷ cells/mL with greater than 95% viability. Suspension cultures can be maintained, without refeeding, for more than 10 days. Sf9 and Sf21 cells have been carried for more than 20 passages in EX-CELL 420 with no loss of viability. Protein expression and virus production are improved over serum-containing media.
Application:	EX-CELL 420 is a complete medium developed and optimized for the serum-free growth of Sf9 and Sf21 insect cell lines. Cells can be subcultured directly into EX-CELL 420 from serum-free or seum-supplemented media without adaptation. Cultures in EX-CELL 420 routinely reach cell densities greater than 1 × 10⁷ cells/mL with greater than 95% viability. Suspension cultures can be maintained, without refeeding, for more than 10 days. Sf9 and Sf21 cells have been carried for more than 20 passages in EX-CELL 420 with no loss of viability. Protein expression and virus production are improved over serum-containing media.
Legal Information:	EX-CELL is a registered trademark of Merck KGaA, Darmstadt, Germany