Phalloidin-Atto 490LS
SIGMA/14479 - suitable for fluorescence
Synonym: Atto 490LS-Phalloidin
Product Type: Chemical
λ | in acetonitrile: water (1:1) |
assay | ≥75.0% (HPLC) |
manufacturer/tradename | ATTO-TEC GmbH |
mol wt | Mw 1466 g/mol |
Quality Level | 100 |
storage temp. | −20°C |
suitability | suitable for fluorescence |
UV absorption | λ: 495-501 nm Amax |
General description: | Atto 490LS is a new fluorescent label featuring an extraordinary large Stokes-Shift of 165 nm. Thus the emission spectrum is almost completely separated from its absorption spectrum, making the dye highly suitable for multiplexing experiments, in particular in combination with Atto 488 and Atto 514. Atto 490LS is very hydrophillic and shows excellent water solubility. The dye exhibits a relatively high fluorescence quantum yield, which is only slightly reduced after conjugation to biomolecules, e.g. proteins, even at high degrees of labeling (DOL). Atto 490LS is an anionic dye. After conjugation to a substrate the dye carries a net electrical charge of -1. Phalloidin, a bicyclic heptapeptide, is a very strong binding reagent to actin. Fluorescent labeled phalloidin has become a useful tool to investigate the distribution of F-actin within the cytoskeleton of cells by fluorescence microscopy. find more information here |
Legal Information: | This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing. |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 3 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Purity | ≥75.0% (HPLC) |
Storage Temp. | −20°C |
UNSPSC | 12352200 |