Synonym: 2′-(4-Ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5′-bi-1H-benzimidazole trihydrochloride; HOE 33342; Hoechst 33342; bisBenzimide
CAS Number: 875756-97-1
Empirical Formula (Hill Notation): C27H28N6O · 3HCl · xH2O
Molecular Weight: 561.93 (anhydrous basis)
EC Number: 245-691-1
MDL Number: MFCD00012678
Linear Formula: C27H28N6O · 3HCl · xH2O
Product Type: Chemical
Immunofluorescence bisBenzimide H 33342 trihydrochloride: Cat. No. 14533: Septins are small GTPases that localize in a filamentous pattern in cells. Treatment of cultured Human podocytes with cytochalasin D disrupts the filamentous organization of the actin cytoskeleton, as visualized by phalloidin staining (red). In cytochalasin D-treated podocytes, the normally linear septin 7 filaments (green) curl and form ring-like structures, indicating that septin 7 localization depends on an intact actin cytoskeleton. The nucleus is visualized with DAPI (blue). Submitted by A. Wasik, University of Helsinki.
| assay |
≥97.0% (HPLC) |
| fluorescence |
λex 340 nm; λem 510 nm |
| |
λex 355 nm; λem 465 nm in TE buffer; DNA |
| grade |
for fluorescence |
| InChI |
1S/C29H32N6O.3ClH/c1-3-36-25-8-4-20(5-9-25)28-30-18-22-16-21(6-10-26(22)32-28)29-31-19-23-17-24(7-11-27(23)33-29)35-14-12-34(2)13-15-35;;;/h4-11,16-17H,3,12-15,18-19H2,1-2H3,(H,30,32)(H,31,33);3*1H |
| InChI key |
FYEVKHPLBHLWHK-UHFFFAOYSA-N |
| Quality Level |
200  |
| SMILES string |
Cl[H].Cl[H].Cl[H].CCOc1ccc(cc1)C2=NCc3cc(ccc3N2)C4=NCc5cc(ccc5N4)N6CCN(C)CC6 |
| storage temp. |
2-8°C |
| Application: |
bisBenzimide H 33342 trihydrochloride can be used to study biochemicals and reagents, fluorescent probes, labels, particles and stains, luminescent compounds and detection and wavelength index. bisBenzimide H 33342 trihydrochloride, Hoechst 33342, has been used in a study to investigate the effects of inorganic and organic arsenic compounds on human T-lymphoblastoid leukemia cells. Hoechst 33342 has also been used in a study to examine the effects of 9 calcium antagonists on ABCG2/BCRP-mediated resistance and transport in HeLa and SN-38-resistant HeLa (HeLa/SN100) cells, overexpressing ABCG2/BCRP. |
| Application: |
Useful for staining DNA, chromosomes and nuclei. May be used for fluorescence microscopy or flow cytometry.
Excitation max. = 346 nm
Emission max. = 460 nm |
| Biochem/physiol Actions: |
Membrane-permeable, fluorescent DNA stains with low cytotoxicity that intercalate in A-T regions of DNA. |
| Other Notes: |
Suitable for vital DNA staining of a variety of cell types; As a probe of membrane permeability in mammalian cells |
| Packaging: |
Bottomless glass bottle. Contents are inside inserted fused cone. |
| Hazard Codes |
Xn |
| Risk Statements |
22-37/38 |
| Safety Statements |
22-36/37 |
| RIDADR |
NONH for all modes of transport |
| WGK Germany |
WGK 3 |
| Flash Point(F) |
Not applicable |
| Flash Point(C) |
Not applicable |
| Purity |
≥97.0% (HPLC) |
| Storage Temp. |
2-8°C |
| UNSPSC |
12161505 |
