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bisBenzimide H 33342 trihydrochloride

SIGMA/14533 - suitable for fluorescence, ≥97.0% (HPLC)

Synonym: 2′-(4-Ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5′-bi-1H-benzimidazole trihydrochloride; HOE 33342; Hoechst 33342; bisBenzimide

CAS Number: 875756-97-1
Empirical Formula (Hill Notation): C27H28N6O · 3HCl · xH2O
Molecular Weight: 561.93 (anhydrous basis)
EC Number: 245-691-1
MDL Number: MFCD00012678
Linear Formula: C27H28N6O · 3HCl · xH2O
Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-14533-100MG 100 mg
 $197.00
1/EA		 Add To Favorites
Immunofluorescence bisBenzimide H 33342 trihydrochloride: Cat. No. 14533: Septins are small GTPases that localize in a filamentous pattern in cells. Treatment of cultured Human podocytes with cytochalasin D disrupts the filamentous organization of the actin cytoskeleton, as visualized by phalloidin staining (red). In cytochalasin D-treated podocytes, the normally linear septin 7 filaments (green) curl and form ring-like structures, indicating that septin 7 localization depends on an intact actin cytoskeleton. The nucleus is visualized with DAPI (blue). Submitted by A. Wasik, University of Helsinki.

 

assay ≥97.0% (HPLC)
fluorescence λex 340 nm; λem 510 nm
  λex 355 nm; λem 465 nm in TE buffer; DNA
InChI 1S/C29H32N6O.3ClH/c1-3-36-25-8-4-20(5-9-25)28-30-18-22-16-21(6-10-26(22)32-28)29-31-19-23-17-24(7-11-27(23)33-29)35-14-12-34(2)13-15-35;;;/h4-11,16-17H,3,12-15,18-19H2,1-2H3,(H,30,32)(H,31,33);3*1H
InChI key FYEVKHPLBHLWHK-UHFFFAOYSA-N
Quality Level 200 
SMILES string Cl[H].Cl[H].Cl[H].CCOc1ccc(cc1)C2=NCc3cc(ccc3N2)C4=NCc5cc(ccc5N4)N6CCN(C)CC6
storage temp. 2-8°C
suitability suitable for fluorescence
Application: bisBenzimide H 33342 trihydrochloride can be used to study biochemicals and reagents, fluorescent probes, labels, particles and stains, luminescent compounds and detection and wavelength index. bisBenzimide H 33342 trihydrochloride, Hoechst 33342, has been used in a study to investigate the effects of inorganic and organic arsenic compounds on human T-lymphoblastoid leukemia cells. Hoechst 33342 has also been used in a study to examine the effects of 9 calcium antagonists on ABCG2/BCRP-mediated resistance and transport in HeLa and SN-38-resistant HeLa (HeLa/SN100) cells, overexpressing ABCG2/BCRP.
Application: Useful for staining DNA, chromosomes and nuclei. May be used for fluorescence microscopy or flow cytometry.
Excitation max. = 346 nm
Emission max. = 460 nm
Biochem/physiol Actions: Membrane-permeable, fluorescent DNA stains with low cytotoxicity that intercalate in A-T regions of DNA.
Other Notes: Suitable for vital DNA staining of a variety of cell types; As a probe of membrane permeability in mammalian cells
Packaging: Bottomless glass bottle. Contents are inside inserted fused cone.
Hazard Codes Xn
Risk Statements 22-37/38
Safety Statements 22-36/37
RIDADR NONH for all modes of transport
WGK Germany WGK 3
Flash Point(F) Not applicable
Flash Point(C) Not applicable
Purity ≥97.0% (HPLC)
Storage Temp. 2-8°C
UNSPSC 12161505

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