2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein tetrakis(acetoxymethyl) ester
SIGMA/14562 - BioReagent, for fluorescence
Synonym: BCECF-AM
Empirical Formula (Hill Notation): C39H36O19
Molecular Weight: 808.69
MDL Number: MFCD01862636
Linear Formula: C39H36O19
Product Type: Chemical
| fluorescence | λex 482 nm; λem 528 nm in 0.1 M Tris pH 8.0 (esterase) |
| form | solid |
| grade | for fluorescence |
| product line | BioReagent |
| Quality Level | 100  |
| solubility | acetonitrile: soluble |
| DMF: soluble | |
| DMSO: soluble | |
| storage temp. | 2-8°C |
| Application: | An uncharged, nonfluorescent molecule that is useful for noninvasive bulk loading of cell suspension because it is capable of permeating the cell membrane. Cellular nonspecific esterases cleave its lipophilic blocking groups, resulting in a charged, fluorescent form of the molecule. Compared to the parent compound, this charged derivative leaks out of the cell at a much slower rate. Utilized for pH measurements in perfused tissues, intercellular spaces, mammalian cells, plant cells, bacteria, and yeast. Also, employed to investigate in assays for cellular functional properties such as adhesion, multi-drug resistance, viability and cytotoxicity, apoptosis and chemotaxis. |
| Application: | Cell-permeable ester of BCECF that, on hydrolysis by cytosolic esterases, yields the intracellularly trapped pH-indicator BCECF. Allows simultaneous recording of cell volume changes and intracellular pH in single osteosarcoma cells. |
| Packaging: | Bottomless glass bottle. Contents are inside inserted fused cone. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352108 |