Human Skeletal Muscle Cells: HSkMC, adult

SIGMA/150-05A

Synonym: Human Muscle Cell Line

Product Type: Product-on-demand

Catalog Number	PKG Qty.	Price	Quantity


45-150-05A	1 each	$1000.00 1/EAAdd To Favorites		Add To Favorites

Cell Applications : Human Skeletal Muscle Cells, HSkMC (left) and fused HSkMC (fetal) immunolabeled for desmin (green) (right). Nuclei are visualized with PI (red).

biological source	human skeletal muscle (normal limb)
growth mode	Adherent
karyotype	2n = 46
manufacturer/tradename	Cell Applications, Inc
morphology	Skeletal muscle
packaging	pkg of 500,000 cells
Quality Level	100
relevant disease(s)	muscular dystrophy
shipped in	dry ice
storage temp.	−196°C
technique(s)	cell culture \| mammalian: suitable

Application:	lipid and glucose metabolism, exercise, insulin action, cell signaling
Cell Line Origin:	Muscle (Limb)
Components:	Basal Medium containing 10% FBS & 10% DMSO
Disclaimer:	RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
General description:	Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details. Human Skeletal Muscle Cells (HSkMC) are isolated from the skeletal muscle of limbs from adult or fetal donors. Human Skeletal Muscle Cells can undergo differentiation to exhibit actin and myosin myofilaments. HSkMC from Cell Applications, Inc. provide a useful model system to study many aspects of muscular function and disease.Skeletal Muscle Cells play an instrumental role in the glucose metabolism and diabetes, therefore select HSkMC lots undergo an additional set of characterization at Cell Applications, Inc. to demonstrate AMPK signaling and responsiveness to insulin stimulation.
Preparation Note:	• 2nd passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO • Can be cultured at least 15 doublings
Subculture Routine:	Please refer to the HSkMC Culture Protocol .