Human Skeletal Muscle Cells: HSkMC, fetal
SIGMA/150-05F
Product Type: Product-on-demand
Cell Applications : Human Skeletal Muscle Cells, HSkMC (left) and fused HSkMC (fetal) immunolabeled for desmin (green) (right). Nuclei are visualized with PI (red).
| biological source | human skeletal muscle (normal limb) |
| growth mode | Adherent |
| karyotype | 2n = 46 |
| manufacturer/tradename | Cell Applications, Inc |
| morphology | Skeletal muscle |
| packaging | pkg of 500,000 cells |
| Quality Level | 100  |
| relevant disease(s) | muscular dystrophy |
| shipped in | dry ice |
| storage temp. | −196°C |
| technique(s) | cell culture | mammalian: suitable |
| Application: | lipid and glucose metabolism, exercise, insulin action, cell signaling |
| Cell Line Origin: | Muscle (Limb) |
| Components: | Basal Medium containing 10% FBS & 10% DMSO |
| General description: | Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details. Human Skeletal Muscle Cells (HSkMC) are isolated from the skeletal muscle of limbs from adult or fetal donors. Human Skeletal Muscle Cells can undergo differentiation to exhibit actin and myosin myofilaments. HSkMC provide a useful model system to study many aspects of muscular function and disease. Skeletal Muscle Cells play an instrumental role in the glucose metabolism and diabetes, therefore select HSkMC lots undergo an additional set of characterization to demonstrate AMPK signaling and responsiveness to insulin stimulation. |
| Preparation Note: | • 3rd passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO • Can be cultured at least 15 doublings |
| Subculture Routine: | Please refer to the HSkMC Culture Protocol . |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −196°C |
| UNSPSC | 12352200 |