BRILA MUG Broth
SIGMA/16016 - suitable for microbiology, NutriSelect® Basic
Product Type: Chemical
| application(s) | agriculture environmental food and beverages water monitoring |
| microbiology | |
| composition | 4-methylumbelliferyl-β-D- |
| brilliant green, 0.133 g/L | |
| lactose, 10 g/L | |
| ox-bile (dried), 20 g/L | |
| final pH | 7.2±0.2 (25 °C) |
| form | powder |
| manufacturer/tradename | NutriSelect® Basic |
| Quality Level | 200  |
| shelf life | limited shelf life, expiry date on the label |
| sterility | non-sterile |
| suitability | selective and differential for Citrobacter spp. |
| selective and differential for Escherichia coli | |
| selective and differential for Lactobacillus spp. | |
| selective and differential for Staphylococcus spp. | |
| selective and differential for coliforms |
| Application: | In the detection of E. coli and coliforms. Bile and brilliant green extensively inhibit the growth of accompanying flora, in particular gram-positive microorganisms. The presence of E. coli results in fluorescence in the UV. A positive indole test and possibly gas formation from lactose fermentation provide confirmation. The broth can be used in conjunction with the MPN method for E. coli and coliform enumeration in the water of bathing areas. |
| Legal Information: | NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Other Notes: | Screening of bathing-water for coliforms according to EC directive |
| Preparation Note: | Dissolve 41 g in 1 litre distilled water and pour into tubesfitted with Durham tubes if necessary. Autoclave at 121°C. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| UNSPSC | 41171606 |