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5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt

SIGMA/16670 - ≥95.0% (HPLC)

Synonym: BCIP® p-toluidine salt; X-phosphate p-toluidine salt

CAS Number: 6578-06-9
Empirical Formula (Hill Notation): C8H6BrClNO4P · C7H9N
Molecular Weight: 433.62
EC Number: 229-506-1
MDL Number: MFCD00040636
Linear Formula: C8H6BrClNO4P · C7H9N
Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-16670-100MG 100 mg
 $147.00
1/EA		 Add To Favorites
45-16670-1G 1 g
 $778.00
1/EA		 Add To Favorites

 

assay ≥95.0% (HPLC)
form powder
InChI 1S/C8H6BrClNO4P.C7H9N/c9-4-1-2-5-7(8(4)10)6(3-11-5)15-16(12,13)14;1-6-2-4-7(8)5-3-6/h1-3,11H,(H2,12,13,14);2-5H,8H2,1H3
InChI key QEIFSLUFHRCVQL-UHFFFAOYSA-N
Quality Level 200 
SMILES string Cc1ccc(N)cc1.OP(O)(=O)Oc2c[nH]c3ccc(Br)c(Cl)c23
storage temp. 2-8°C
Application: For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry.
BCIP is provided in two salt forms: the disodium salt which is soluble in water and the p-toluidine form which is soluble in dimethylformamide. These salt forms may be used to prepare a stock solution that in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color. For added convenience, a mixture of BCIP and NBT is provided in an easily dissolvable tablet form or as a ready-to-use liquid.
Application: For the colorimetric detection of alkaline phosphatase-labeled molecules.
General description: Histochemical substrate for alkaline phosphatase.
Legal Information: BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany
Packaging: 1, 5 g in poly bottle
Packaging: 100 mg in poly bottle
Hazard Codes Xi
Risk Statements 36/37/38
Safety Statements 26
RIDADR NONH for all modes of transport
WGK Germany WGK 3
Flash Point(F) Not applicable
Flash Point(C) Not applicable
Purity ≥95.0% (HPLC)
Storage Temp. 2-8°C
UNSPSC 12352204

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