Atto 425 NHS ester

SIGMA/16805 - BioReagent, suitable for fluorescence, ≥90% (HPLC)

Synonym: Atto 425-N-hydroxysuccinimide ester

CAS Number: 892156-28-4
Empirical Formula (Hill Notation): C₂₆H₃₀N₂O₈
Molecular Weight: 498.53
MDL Number: MFCD04974819
Linear Formula: C₂₆H₃₀N₂O₈
Product Type: Chemical

Catalog Number	PKG Qty.	Price	Quantity


45-16805-1MG-F	1 mg	$93.30 1/EAAdd To Favorites		Add To Favorites
45-16805-5MG-F	5 mg	$450.00 1/EAAdd To Favorites		Add To Favorites

λ	in ethanol (with 0.1% trifluoroacetic acid)
assay	≥90% (degree of coupling)
	≥90% (HPLC)
InChI	1S/C26H30N2O8/c1-5-34-24(32)18-12-16-11-17-15(2)14-26(3,4)27(19(17)13-20(16)35-25(18)33)10-6-7-23(31)36-28-21(29)8-9-22(28)30/h11-13,15H,5-10,14H2,1-4H3
InChI key	PUEQEMDTFPYCDY-UHFFFAOYSA-N
manufacturer/tradename	ATTO-TEC GmbH
product line	BioReagent
Quality Level	100
SMILES string	CCOC(=O)C1=Cc2cc3C(C)CC(C)(C)N(CCCC(=O)ON4C(=O)CCC4=O)c3cc2OC1=O
solubility	DMF: soluble
	DMSO: soluble
	H₂O: soluble
storage temp.	−20°C
suitability	suitable for fluorescence
UV absorption	λ: 422-428 nm A_max

Application:	Atto 425 NHS ester is used in the development of bifunctional fluorescent, inorganic micro-partices (Zeolite L crystals) designed for targeting or imaging mutliple components . Reacts with amino groups or amine modified oliognucleotides to form a stable amine bond. Utilized as an essential component of molecular beacons, which were designed for use in real-time PCR assays to allow for rapid and accurate identification of gram-negative bacteria . When conjugated with benzylguanine, the fluorescence of Atto 425-NHS is quenched but becomes fluorescent upon labeling of the SNAP-tag fusion protein, making this conjugate an ideal candidate for experiments in living cells . Used as one of the donor probes in three-color FRET experiments designed to investigate the kinetics of RNA-protein complex formation .
Application:	Atto fluorescent labels are designed for high sensitivity applications, including single molecule detection. Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation.
General description:	Atto 425 is a member of the commercially available coumarin derivatives.
Legal Information:	This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.
Other Notes:	Study of stability during one- and two-photon excitation in fluorescence correlation spectroscopy, FCS; employed in triple-color coincidence analysis.