Atto 425 NHS ester
SIGMA/16805 - BioReagent, suitable for fluorescence, ≥90% (HPLC)
Synonym: Atto 425-
CAS Number: 892156-28-4
Empirical Formula (Hill Notation): C26H30N2O8
Molecular Weight: 498.53
MDL Number: MFCD04974819
Linear Formula: C26H30N2O8
Product Type: Chemical
| λ | in ethanol (with 0.1% trifluoroacetic acid) |
| assay | ≥90% (degree of coupling) |
| ≥90% (HPLC) | |
| InChI | 1S/C26H30N2O8/c1-5-34-24( |
| InChI key | PUEQEMDTFPYCDY-UHFFFAOYSA |
| manufacturer/tradename | ATTO-TEC GmbH |
| product line | BioReagent |
| Quality Level | 100  |
| SMILES string | CCOC(=O)C1=Cc2cc3C(C)CC(C |
| solubility | DMF: soluble |
| DMSO: soluble | |
| H2O: soluble | |
| storage temp. | −20°C |
| suitability | suitable for fluorescence |
| UV absorption | λ: 422-428 nm Amax |
| Application: | Atto 425 NHS ester is used in the development of bifunctional fluorescent, inorganic micro-partices (Zeolite L crystals) designed for targeting or imaging mutliple components . Reacts with amino groups or amine modified oliognucleotides to form a stable amine bond. Utilized as an essential component of molecular beacons, which were designed for use in real-time PCR assays to allow for rapid and accurate identification of gram-negative bacteria . When conjugated with benzylguanine, the fluorescence of Atto 425-NHS is quenched but becomes fluorescent upon labeling of the SNAP-tag fusion protein, making this conjugate an ideal candidate for experiments in living cells . Used as one of the donor probes in three-color FRET experiments designed to investigate the kinetics of RNA-protein complex formation . |
| Application: | Atto fluorescent labels are designed for high sensitivity applications, including single molecule detection. Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation. |
| General description: | Atto 425 is a member of the commercially available coumarin derivatives. |
| Legal Information: | This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing. |
| Other Notes: | Study of stability during one- and two-photon excitation in fluorescence correlation spectroscopy, FCS; employed in triple-color coincidence analysis. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥90% (HPLC); ≥90% (degree of coupling) |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |