Atto 495
SIGMA/16951 - BioReagent, suitable for fluorescence, ≥98.0% (HPCE)
MDL Number: MFCD07370099
Product Type: Chemical
| assay | ≥98.0% (HPCE) |
| form | powder |
| InChI | 1S/C21H25N3O2.ClHO4/c1-22 |
| InChI key | KCYUESOHZRSRGP-UHFFFAOYSA |
| manufacturer/tradename | ATTO-TEC GmbH |
| product line | BioReagent |
| Quality Level | 100  |
| SMILES string | [O-]Cl(=O)(=O)=O.CN(C)c1c |
| solubility | acetone: soluble |
| DMF: soluble | |
| DMSO: soluble | |
| H2O: soluble | |
| storage temp. | −20°C |
| suitability | suitable for fluorescence |
| Application: | Atto 495 is a lipophilic fluorescent compound, which has served as the neutral analyte in the model, along with SDS as the charged micelle acting as a carrier to transport this solute in an electric field. Atto 495 was included as fluorescence EOF marker for chip electrophoresis. This reagent is zwitterionic at pH 8.3 and thus migrates as a neutral substance under the proper conditions. |
| Application: | Atto fluorescent labels are designed for high sensitivity applications, including single molecule detection. Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation. |
| General description: | Atto 495 is a new label for proteins and oligo-nucleotides with high molecular absorption (80.000) and quantum yield (0.45) as well as sufficient Stoke′s shift between excitation and emission maximum. Atto 495 is characterized by a high photostability and thermostability. It is slightly hydrophilic, and solubility in polar solvents as DMF or DMSO is high. |
| Legal Information: | This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥98.0% (HPCE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |