Calcein-AM
SIGMA/17783 - BioReagent, suitable for fluorescence, ≥95.0% (HPLC)
Synonym: Calcein acetoxymethyl ester; Calcein O,O′-diacetate tetrakis(acetoxymethyl) ester
CAS Number: 148504-34-1
Empirical Formula (Hill Notation): C46H46N2O23
Molecular Weight: 994.86
MDL Number: MFCD05861516
Linear Formula: C46H46N2O23
Product Type: Chemical
assay | ≥95.0% (HPLC) |
fluorescence | λex 496 nm; λem 516 nm in 0.1 M Tris pH 8.0, esterase; Ca2+ |
InChI | 1S/C46H46N2O23/c1-25(49)6 |
InChI key | XKFSBWQWNMZWFA-UHFFFAOYSA |
product line | BioReagent |
Quality Level | 100 |
SMILES string | CC(=O)OCOC(=O)CN(CC(=O)OC |
storage temp. | −20°C |
suitability | suitable for fluorescence |
Application: | Calcein-AM has been used in flow cytometry. |
Application: | Non-fluorescent cell permeable derivative of calcein, becomes fluorescent on hydrolysis. Used as a neutral substrate for multidrug resistance protein, MRP; employed in tumor research. |
General description: | Liposomes contain a self-quenching fluorescent dye called calcein. It is a fluorescent dye with excitation and emission wavelengths of 495/515 nm, respectively. It self-quenches at concentrations above 70mM and is commonly used as an indicator of lipid vesicle leakage. The leakage rate is calculated on the basis of the fluorescence increment as the entrapped calcein is leaked out of the liposomal compartment. It is used as a complexometric indicator for titration of calcium ions with EDTA, and for fluorometric determination of calcium. |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 3 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Purity | ≥95.0% (HPLC) |
Storage Temp. | −20°C |
UNSPSC | 12352200 |