Atto 565 maleimide

SIGMA/18507 - BioReagent, suitable for fluorescence

Empirical Formula (Hill Notation): C₃₇H₃₇ClN₄O₁₀
Molecular Weight: 733.16
MDL Number: MFCD04040675
Linear Formula: C₃₇H₃₇ClN₄O₁₀
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-18507-1MG-F	1 mg	$1740.00 1/EAAdd To Favorites	Add To Favorites

λ	(ethanol with 0.1% trifluoroacetic acid)
assay	>90.0% (HPLC)
fluorescence	λ_ex 563 nm; λ_em 592 nm in 0.1 M phosphate pH 7.0
form	powder
manufacturer/tradename	ATTO-TEC GmbH
product line	BioReagent
Quality Level	200
storage temp.	−20°C
suitability	corresponds for coupling to thiols
	suitable for fluorescence
transmittance	254 nm
	565 nm
UV absorption	λ: 560-566 nm A_max

Application:

Atto 565 is a new label with high molecular absorption (120.000) and quantum yield (0.9) as well as sufficient stoke′s shift (excitation maximum 563 nm, emission maximum 592 nm). Due to an insignificant triplet formation rate it is well suited for single molecule detection applications. Maleimides are well suited for coupling to thiol groups. This is similar to iodacetamides, but maleimides do react more thiol selective. They do not show significant reaction with histidine or methionine. Hydrolysis of maleimides to a mixture of isomeric nonreactive maleamic acids can compete significantly with thiol modification, particularly above pH 8. Maleimides may be used for labeling of amines, which usually requires a higher pH than reaction of maleimides with thiols.

Legal Information:

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Purity	>90.0% (HPLC)
Storage Temp.	−20°C
UNSPSC	12352111