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Atto 565 maleimide

SIGMA/18507 - BioReagent, suitable for fluorescence

Empirical Formula (Hill Notation): C37H37ClN4O10
Molecular Weight: 733.16
MDL Number: MFCD04040675
Linear Formula: C37H37ClN4O10
Product Type: Chemical

Catalog Number PKG Qty. Price Quantity
45-18507-1MG-F 1 mg
$1680.00
1/EA
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λ (ethanol with 0.1% trifluoroacetic acid)
assay >90.0% (HPLC)
fluorescence λex 563 nm; λem 592 nm in 0.1 M phosphate pH 7.0
form powder
manufacturer/tradename ATTO-TEC GmbH
product line BioReagent
Quality Level 200 
storage temp. −20°C
suitability corresponds for coupling to thiols
  suitable for fluorescence
transmittance 254 nm
  565 nm
UV absorption λ: 560-566 nm Amax
Application: Atto 565 is a new label with high molecular absorption (120.000) and quantum yield (0.9) as well as sufficient stoke′s shift (excitation maximum 563 nm, emission maximum 592 nm). Due to an insignificant triplet formation rate it is well suited for single molecule detection applications. Maleimides are well suited for coupling to thiol groups. This is similar to iodacetamides, but maleimides do react more thiol selective. They do not show significant reaction with histidine or methionine. Hydrolysis of maleimides to a mixture of isomeric nonreactive maleamic acids can compete significantly with thiol modification, particularly above pH 8. Maleimides may be used for labeling of amines, which usually requires a higher pH than reaction of maleimides with thiols.
Legal Information: This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.
RIDADR NONH for all modes of transport
WGK Germany WGK 3
Flash Point(F) Not applicable
Flash Point(C) Not applicable
Purity >90.0% (HPLC)
Storage Temp. −20°C
UNSPSC 12352111

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