Atto 565 maleimide
SIGMA/18507 - BioReagent, suitable for fluorescence
Empirical Formula (Hill Notation): C37H37ClN4O10
Molecular Weight: 733.16
MDL Number: MFCD04040675
Linear Formula: C37H37ClN4O10
Product Type: Chemical
| λ | (ethanol with 0.1% trifluoroacetic acid) |
| assay | >90.0% (HPLC) |
| fluorescence | λex 563 nm; λem 592 nm in 0.1 M phosphate pH 7.0 |
| form | powder |
| manufacturer/tradename | ATTO-TEC GmbH |
| product line | BioReagent |
| Quality Level | 200  |
| storage temp. | −20°C |
| suitability | corresponds for coupling to thiols |
| suitable for fluorescence | |
| transmittance | 254 nm |
| 565 nm | |
| UV absorption | λ: 560-566 nm Amax |
| Application: | Atto 565 is a new label with high molecular absorption (120.000) and quantum yield (0.9) as well as sufficient stoke′s shift (excitation maximum 563 nm, emission maximum 592 nm). Due to an insignificant triplet formation rate it is well suited for single molecule detection applications. Maleimides are well suited for coupling to thiol groups. This is similar to iodacetamides, but maleimides do react more thiol selective. They do not show significant reaction with histidine or methionine. Hydrolysis of maleimides to a mixture of isomeric nonreactive maleamic acids can compete significantly with thiol modification, particularly above pH 8. Maleimides may be used for labeling of amines, which usually requires a higher pH than reaction of maleimides with thiols. |
| Legal Information: | This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | >90.0% (HPLC) |
| Storage Temp. | −20°C |
| UNSPSC | 12352111 |