CHAPS 100 mM solution

SIGMA/19899

Synonym: CHAPS solution

CAS Number: 75621-03-3
Empirical Formula (Hill Notation): C₃₂H₅₈N₂O₇S
Molecular Weight: 614.88
MDL Number: MFCD00012116
Linear Formula: C₃₂H₅₈N₂O₇S
Product Type: Chemical

Catalog Number	PKG Qty.	Price	Quantity


45-19899-1ML-F	1 mL	$75.10 1/EAAdd To Favorites		Add To Favorites

CMC	8 mM
concentration	100 mM in H₂O
density	1.01 g/mL at 20 °C
description	zwitterionic
form	liquid
InChI	1S/C32H58N2O7S/c1-21(8-11-29(38)33-14-6-15-34(4,5)16-7-17-42(39,40)41)24-9-10-25-30-26(20-28(37)32(24,25)3)31(2)13-12-23(35)18-22(31)19-27(30)36/h21-28,30,35-37H,6-20H2,1-5H3,(H-,33,38,39,40,41)/t21-,22?,23-,24-,25+,26+,27-,28+,30+,31+,32-/m1/s1
InChI key	UMCMPZBLKLEWAF-RFCNGIAKSA-N
mol wt	614.88 g/mol
Quality Level	100
SMILES string	C[C@H](CCC(=O)NCCC[N+](C)(C)CCCS([O-])(=O)=O)[C@H]1CC[C@H]2[C@@H]3[C@H](O)CC4C[C@H](O)CC[C@]4(C)[C@H]3C[C@H](O)[C@]12C
storage temp.	2-8°C
technique(s)	protein quantification: suitable
transition temp	cloud point >100 °C

Application:	CHAPS 100 mM solution has been used as a component of lysis buffer for the extraction of proteins from goat endometrial epithelial cells (EECs) and liver tissue.
Biochem/physiol Actions:	3-[(3-cholamidopropyl) dimethylammonio]-1-propanesulfonate (CHAPS) exhibits the properties of both sulfobetaine-type surfactants and bile salts. It is involved in the protein extraction process for proteomic analysis and reconstitution of membrane proteins into lipid vesicles (proteoliposomes). CHAPS is broadly used in biochemistry for membrane protein and receptor isolation and purification. It is conveniently used in pharmaceuticals and medicines due to its biocompatibility, noncharged character, nondenaturing, and disaggregating properties.
General description:	3-[(3-cholamidopropyl) dimethylammonio]-1-propanesulfonate (CHAPS) is a biocompatible surfactant. It is a derivative of cholic acid comprising a hydrophobic cholesterol-like skeleton possessing three hydroxyl groups and a zwitterionic aliphatic tail.