CD123 (6H6) Mouse Monoclonal Antibody

SIGMA/198M-1

Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-198M-14	0.1 mL	$0.00 1/EA CALL FOR PRICEAdd To Favorites	Add To Favorites
45-198M-15	0.5 mL	$0.00 1/EA CALL FOR PRICEAdd To Favorites	Add To Favorites
45-198M-16	1 mL	$0.00 1/EA CALL FOR PRICEAdd To Favorites	Add To Favorites
45-198M-17	1 mL	$0.00 1/EA CALL FOR PRICEAdd To Favorites	Add To Favorites
45-198M-18	7 mL	$0.00 1/EA CALL FOR PRICEAdd To Favorites	Add To Favorites

Immunohistochemistry CD123 (6H6) on plasmacytoid dendritic cell neoplasm

antibody form	culture supernatant
antibody product type	primary antibodies
biological source	mouse
clone	6H6, monoclonal
conjugate	unconjugated
control	blastic plasmacytoid dendritic cell neoplasm
description	For In Vitro Diagnostic Use in Select Regions (See Chart)
form	buffered aqueous solution
isotype	IgG1κ
manufacturer/tradename	Cell Marque^™
packaging	bottle of 1.0 mL predilute (198M-17)
	bottle of 7.0 mL predilute (198M-18)
	vial of 0.1 mL concentrate (198M-14)
	vial of 0.5 mL concentrate (198M-15)
	vial of 1.0 mL concentrate (198M-16)
Quality Level	100
	500
shipped in	wet ice
species reactivity	human
storage temp.	2-8°C
technique(s)	immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:25-1:100
visualization	cytoplasmic

General description:

Blastic plasmacytoid dendritic cell neoplasm (BPDCN), previously known as CD4+/CD56+ hematodermic neoplasm or blastic NK-cell lymphoma, is a malignant neoplasm composed of immature hematopoietic precursors of plasmacytoid dendritic cells. The most frequent manifestation is a skin lesion, bone marrow involvement, and regional lymphadenopathy. Myeloid leukemia cutis (LC), myeloid sarcoma, and large aggressive B cell lymphomas should be differentiated from BPDCN. Recently, it has been reported that these entities can be distinguished by using immunohistochemistry (IHC) in paraffin-embedded tissue sections. In this study, 23 myeloid LC and 12 BPDCN cases were evaluated using a panel of antibodies against CD123, TCL1, CD4, CD56, MPO and CD33; with results as follows: anti-CD123 stained 4 cases (17%) of myeloid LC and 10 cases (83%) of BPDCN; anti-TCL-1 stained 2 cases (9%) of myeloid LC and 9 (82%) of 11 cases of BPDCN; anti-CD4 stained 2 cases (9%) of LC and all 12 cases (100%) of BPDCN; anti-CD56 stained 12 cases (52%) of LC and all 12 cases (100%) of BPDCN; anti-myeloperoxidase stained 7 cases (30%) of LC and 0 cases (0%) of BPDCN. Anti-CD33 was not helpful; it stained 18 (78%) cases of LC and 11 cases (92%) of BPDCN. The results indicated that a panel that includes antibodies against CD4, CD56, CD123, and TCL-1 can appropriately distinguish between myeloid LC and BPDCN.
CD123 IHC expression has been studied in 157 acute myeloid leukemia (AML) bone marrow biopsies and/or marrow particle preparations, and correlated with the morphologic, immunophenotypic, and cytogenetic features and with the presence of FLT3-ITD and NPM1 mutations. CD123 IHC expression has been seen in 40% of AML, across a wide spectrum of 2008 World Health Organization subtypes and was most frequent within the intermediate risk group. Compared with CD123 IHC negative AML, CD123 IHC positive AML demonstrated higher marrow blast percentages (median 69%), monocytic differentiation (33/63 cases), and CD34 negativity (29/63 cases). 83% (25/30) FLT3-ITD-mutated AML were CD123+ and 62% (18/29) NPM1-mutated cases were CD123 IHC+ (P=0.0052). CD123 IHC+AML presents with characteristic pathologic features, some of which may be related to underlying FLT3-ITD and/or NPM1 mutations.

Legal Information:

Cell Marque is a trademark of Merck KGaA, Darmstadt, Germany

Linkage:

CD123 Positive Control Slides, Product No. 198S External link

, are available for immunohistochemistry (formalin-fixed, paraffin-embedded sections).

Other Notes:

For Technical Service please contact: 800-665-7284 or email: service@cellmarque.com

Physical form:

Solution in Tris Buffer, pH 7.3-7.7, with 1% BSA and <0.1% Sodium Azide

Preparation Note:

Download the IFU specific to your product lot and format External link

Note: This requires a keycode which can be found on your packaging or product label.

Quality:

IVD

RUO

RIDADR	NONH for all modes of transport
WGK Germany	WGK 2
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Storage Temp.	2-8°C
UNSPSC	12352203