5(6)-Carboxyfluorescein diacetate

SIGMA/21879 - BioReagent, suitable for fluorescence, ≥90.0% (HPLC)

Synonym: 5(6)-Carboxy-di-O-acetylfluorescein

CAS Number: 124387-19-5
Empirical Formula (Hill Notation): C₂₅H₁₆O₉
Molecular Weight: 460.39
MDL Number: MFCD00467494
Linear Formula: C₂₅H₁₆O₉
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-21879-25MG-F	25 mg	$82.20 1/EAAdd To Favorites	Add To Favorites
45-21879-100MG-F	100 mg	$275.00 1/EAAdd To Favorites	Add To Favorites

assay	≥90.0% (HPLC)
fluorescence	λ_ex 492 nm; λ_em 517 nm in 0.1 M Tris pH 8.0 (esterase)
form	powder
InChI	1S/2C25H16O9/c1-12(26)31-15-4-7-18-21(10-15)33-22-11-16(32-13(2)27)5-8-19(22)25(18)20-9-14(23(28)29)3-6-17(20)24(30)34-25;1-12(26)31-14-6-8-18-20(10-14)33-21-11-15(32-13(2)27)7-9-19(21)25(18)22-16(23(28)29)4-3-5-17(22)24(30)34-25/h2*3-11H,1-2H3,(H,28,29)
InChI key	SWRGCNMDGRMQGB-UHFFFAOYSA-N
product line	BioReagent
Quality Level	100
SMILES string	CC(=O)Oc1ccc2c(Oc3cc(OC(C)=O)ccc3C24OC(=O)c5ccc(cc45)C(O)=O)c1.CC(=O)Oc6ccc7c(Oc8cc(OC(C)=O)ccc8C79OC(=O)c%10cccc(C(O)=O)c9%10)c6
solubility	DMSO: soluble
storage temp.	2-8°C
suitability	suitable for fluorescence

Application:

5(6)-Carboxyfluorescein diacetate (CFDA) is a dye which is been extensively utilized to label cultured neurons with the essential requirements, in a specific manner.

5(6)- Carboxyfluorescein diacetate (CFDA) is a viability probe that assesses enzymatic activity, which is necessary to activate its fluorescence, and cell-membrane integrity, which is necessary for the retention of their fluorescent product within the cell.

General description:

5(6)-Carboxyfluorescein diacetate or CDFDA is a fluorescent oxidant sensing probe. It is an intracellular probe added to the cells as a pro-substrate, which upon hydrolysis by the esterase enzyme produces the fluorescent product called CDF.

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Purity	≥90.0% (HPLC)
Storage Temp.	2-8°C
UNSPSC	12352108