5(6)-Carboxy-2′,7′-dichlorofluorescein diacetate

SIGMA/21884 - BioReagent, suitable for fluorescence, ≥85% (HPCE)

Synonym: 5&6DH-DA; CDFDA; Carboxy-DCFDA

CAS Number: 127770-45-0
Empirical Formula (Hill Notation): C₂₅H₁₄Cl₂O₉
Molecular Weight: 529.28
MDL Number: MFCD08459585
Linear Formula: C₂₅H₁₄Cl₂O₉
Product Type: Chemical

Catalog Number	PKG Qty.	Price	Quantity


45-21884-100MG	100 mg	$308.00 1/EA		Add To Favorites

Properties
Descriptions
Safety Info.
Basic Data

assay	≥85% (HPCE)
fluorescence	λ_ex 470 nm; λ_em 529 nm in 0.1 M Tris pH 8.0 (esterase)
InChI	1S/2C25H14Cl2O9/c1-10(28)33-21-8-19-15(6-17(21)26)25(14-4-3-12(23(30)31)5-13(14)24(32)36-25)16-7-18(27)22(34-11(2)29)9-20(16)35-19;1-10(28)33-21-8-19-15(6-17(21)26)25(14-5-12(23(30)31)3-4-13(14)24(32)36-25)16-7-18(27)22(34-11(2)29)9-20(16)35-19/h2*3-9H,1-2H3,(H,30,31)
InChI key	LZMPJBOMBCYYMP-UHFFFAOYSA-N
mp	210 °C (dec.) (lit.)
product line	BioReagent
Quality Level	100
SMILES string	CC(=O)Oc1cc2Oc3cc(OC(C)=O)c(Cl)cc3C4(OC(=O)c5cc(ccc45)C(O)=O)c2cc1Cl.CC(=O)Oc6cc7Oc8cc(OC(C)=O)c(Cl)cc8C9(OC(=O)c%10ccc(cc9%10)C(O)=O)c7cc6Cl
solubility	DMF: soluble
	DMSO: soluble
	methanol: soluble
storage temp.	−20°C
suitability	suitable for fluorescence

Analysis Note:	Exc_max: 504 nm (Lit.)
Application:	5(6)-Carboxy-2′,7′-dichlorofluorescein diacetate or Carboxy-DCFDA is used as a cell permeate probe to detect intracellular reactive oxygen species (ROS). The anionic fluorescent dye, 5(6)-Carboxy-2′,7′-dichlorofluorescein diacetate is suitable for studying Multi-Drug Resistance (MDR) by target cells.
General description:	5(6)-Carboxy-2′,7′-dichlorofluorescein diacetate or CDFDA is a fluorescent oxidant sensing probe. It is an intracellular probe added to the cells as a pro-substrate, which upon hydrolysis by the esterase enzyme produces the fluorescent product called CDF.
Other Notes:	Intracellular esterase substrate with low sensitivity of fluorescence to intracellar pH
Packaging:	Bottomless glass bottle. Contents are inside inserted fused cone.

Symbol	GHS07
Signal word	Warning
Hazard statements	H315 - H319 - H335
Precautionary statements	P302 + P352 - P305 + P351 + P338
Hazard Codes	Xi
Risk Statements	36/37/38
Safety Statements	26
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Purity	≥85% (HPCE)
mp	210 °C (dec.) (lit.)
Storage Temp.	−20°C
UNSPSC	12352106

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