5(6)-Carboxyfluorescein diacetate N-succinimidyl ester

SIGMA/21888 - BioReagent, suitable for fluorescence, ≥90% (HPLC)

Synonym: CFSE

CAS Number: 150347-59-4
Empirical Formula (Hill Notation): C₂₉H₁₉NO₁₁
Molecular Weight: 557.46
MDL Number: MFCD00037466
Linear Formula: C₂₉H₁₉NO₁₁
Product Type: Chemical

Catalog Number	PKG Qty.	Price	Quantity


45-21888-25MG-F	25 mg	$426.00 1/EA		Add To Favorites
45-21888-100MG-F	100 mg	$1190.00 1/EA		Add To Favorites

Properties
Descriptions
Safety Info.
Basic Data

assay	≥90% (HPLC)
color	almost white to yellow
description	suitable as fluorogenic substrate for esterases
fluorescence	λ_ex 492 nm; λ_em 517 nm in 0.1 M Tris pH 8.0 (esterase)
form	solid
InChI	1S/2C29H19NO11/c1-14(31)37-17-4-7-21-23(12-17)39-24-13-18(38-15(2)32)5-8-22(24)29(21)20-6-3-16(11-19(20)28(36)40-29)27(35)41-30-25(33)9-10-26(30)34;1-14(31)37-17-4-7-20-23(12-17)39-24-13-18(38-15(2)32)5-8-21(24)29(20)22-11-16(3-6-19(22)28(36)40-29)27(35)41-30-25(33)9-10-26(30)34/h2*3-8,11-13H,9-10H2,1-2H3
InChI key	LZFDYQHOQDFMOA-UHFFFAOYSA-N
mol wt	557.46 g/mol
mp	152-154 °C (lit.)
product line	BioReagent
Quality Level	200
SMILES string	CC(=O)Oc1ccc2c(Oc3cc(OC(C)=O)ccc3C24OC(=O)c5cc(ccc45)C(=O)ON6C(=O)CCC6=O)c1.CC(=O)Oc7ccc8c(Oc9cc(OC(C)=O)ccc9C8%10OC(=O)c%11ccc(cc%10%11)C(=O)ON%12C(=O)CCC%12=O)c7
solubility	DMF: soluble
	DMSO: soluble
storage temp.	−20°C
suitability	suitable for fluorescence

Application:	5(6)-Carboxyfluorescein diacetate N-succinimidyl ester (CFSE) is an amine reactive fluorescent probe used to stain cells, especially immune cells, for analysis in applications such as flow cytometry. Forms green fluorescent conjugates on deacetylation.
Application:	5-Carboxyfluorescein diacetate N-succinimidyl ester has been used: • to label cells for flow cytometry • in lymphocyte proliferation assay • to determine the intracellular or intra-organellar pH in chloroplasts • to label Leishmania amazonensis and Leishmania major
Application:	Amine-reactive label for long term tracing of cells. Forms green fluorescent conjugates on deacetylation.
Biochem/physiol Actions:	5(6)-Carboxyfluorescein diacetate N-succinimidyl ester is used as an indicator for esterase activity.
General description:	5-(and-6)-Carboxyfluorescein diacetate N-succinimidyl ester (CFSE) is a fluorescent dye, which is membrane permeable. It labels cells by covalently binding to intracellular molecules. CFSE is used to track lymphocyte migration and proliferation.
Packaging:	Bottomless glass bottle. Contents are inside inserted fused cone.

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Purity	≥90% (HPLC)
mp	152-154 °C (lit.)
Storage Temp.	−20°C
UNSPSC	12352108

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