Synonym: CFSE
CAS Number: 150347-59-4
Empirical Formula (Hill Notation): C29H19NO11
Molecular Weight: 557.46
MDL Number: MFCD00037466
Linear Formula: C29H19NO11
Product Type: Chemical
assay | ≥90% (HPLC) |
fluorescence | λex 492 nm; λem 517 nm in 0.1 M Tris pH 8.0 (esterase) |
form | solid |
InChI | 1S/2C29H19NO11/c1-14(31)3 |
InChI key | LZFDYQHOQDFMOA-UHFFFAOYSA |
mp | 152-154 °C (lit.) |
product line | BioReagent |
Quality Level | 200 ![]() |
SMILES string | CC(=O)Oc1ccc2c(Oc3cc(OC(C |
solubility | DMF: soluble |
DMSO: soluble | |
storage temp. | −20°C |
suitability | suitable for fluorescence |
Application: | 5(6)-Carboxyfluorescein diacetate N-succinimidyl ester (CFSE) is an amine reactive fluorescent probe used to stain cells, especially immune cells, for analysis in applications such as flow cytometry. Forms green fluorescent conjugates on deacetylation. |
Application: | 5-Carboxyfluorescein diacetate N-succinimidyl ester has been used: • to label cells for flow cytometry • in lymphocyte proliferation assay • to determine the intracellular or intra-organellar pH in chloroplasts • to label Leishmania amazonensis and Leishmania major |
Application: | Amine-reactive label for long term tracing of cells. Forms green fluorescent conjugates on deacetylation. |
Biochem/physiol Actions: | 5(6)-Carboxyfluorescein diacetate N-succinimidyl ester is used as an indicator for esterase activity. |
General description: | 5-(and-6)-Carboxyfluoresc |
Packaging: | Bottomless glass bottle. Contents are inside inserted fused cone. |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 3 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Purity | ≥90% (HPLC) |
mp | 152-154 °C (lit.) |
Storage Temp. | −20°C |
UNSPSC | 12352108 |
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