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Cellulase from Aspergillus niger

SIGMA/22178 - powder, off-white, ~0.8 U/mg

Synonym: 1,4-(1,3:1,4)-β-D-Glucan 4-glucanohydrolase

CAS Number: 9012-54-8
EC Number: 232-734-4
MDL Number: MFCD00081510
Product Type: Chemical

Catalog Number PKG Qty. Price Quantity
45-22178-25G 25 g
$166.00
1/EA
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45-22178-100G 100 g
$551.00
1/EA
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biological source Aspergillus niger
color off-white
form powder
Quality Level 100 
specific activity ~0.8 U/mg
storage temp. 2-8°C
Application: Cellulase from Aspergillus niger has been used:
• in the preparation of the standardized solution to determine absolute cellulase levels
• in phosphate-citric acid buffer for activity assay
• in non-starch polysaccharide degrading enzyme cocktail for in vitro hydrolysis of defatted rice bran and in the approach to improve the nutritive value of poultry feed
Biochem/physiol Actions: Cellulase from Aspergillus niger catalyzes the hydrolysis of endo-1,4-β-D-glycosidic linkages in cellulose, lichenin, barley glucan, and the cellooligosaccharides cellotriose to cellohexaose. It does not cleave cellobiose or p-nitrophenyl-β-D-glucoside. This enzyme will also cleave intact glycosaminoglycan from a core peptide by hydrolyzing the xylosyl serine linkage.
General description: Cellulase is a member of glycoside hydrolase family and is produced by a number of cellulolytic microbes.
Other Notes: View more information on enzymes for complex carbohydrate analysis  at www.sigma-aldrich.com/enzymeexplorer 
Unit Definition: 1 U corresponds to the amount of enzymes which liberates 1 μmol glucose from carboxymethylcellulose per minute at pH 5.0 and 37°C
activity specific activity: ~0.8 U/mg
Storage Temp. 2-8°C
Enzyme Commission (EC) Number 3.2.1.4   ( BRENDA  | IUBMB  )
UNSPSC 12352204

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