Nitrite/Nitrate Assay Kit, colorimetric
SIGMA/23479 - used for detection of nitric oxide metabolites
Synonym: Nitrate/Nitrite Assay Kit, colorimetric
Product Type: Chemical
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| Quality Level | 100  |
| storage temp. | 2-8°C |
| suitability | passes test for application |
| usage | sufficient for 100 assays |
| Application: | Nitric oxide (NO), a gaseous paramagnetic radical, is a very important and versatile messenger in biological systems. NO is synthesized from L-arginine by NO synthase (NOS). It has been identified as an endothelial derived relaxation factor (EDRF) and antiplatelet substance. It serves as a neurotransmitter derived from a neutrophile and a cytotoxic substance from an activated macrophage. Although NO’s molecular action in the biological system is very versatile, the most important role of NO is the activation of guanylate cyclase. The Griess assay is one of the most popular and simplest methods used to detect the NO concentration. The Griess assay’s mechanism is summarized as the azo coupling between diazonium species, which are produced from sulfanilamide with NO2, and naphthylethylenediamine. The NO2/NO3 Assay Kit contains these dyes, a nitrate reductase, an enzyme co-factor, buffer solution and NO2, NO3 solutions as standards. Therefore, total NO metabolites are easily detectable using this kit. The suitable NO2 detection range is from 10 to 100 μM. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H315 - H319 |
| Precautionary statements | P264 - P280 - P302 + P352 - P305 + P351 + P338 - P332 + P313 - P337 + P313 |
| Hazard Codes | Xi |
| Risk Statements | 36/37/38 |
| Safety Statements | 26 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12161503 |
| Components | NaNO2 Standard Solution (100 mM) 1 bottle; NaNO3 Standard Solution (100 mM) 1 bottle; Buffer solution (20 mM, pH 7.6) 1 bottle; Nitrate reductase (lyophilized) 1 bottle; Enzyme Co-factor (lyophilized) 1 bottle; Griess Reagent A 1 bottle; Griess Reagent B 1 bottle |