FLI-1 (MRQ-1) Mouse Monoclonal Antibody

SIGMA/254M-1

Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-254M-14	0.1 mL	$0.00 1/EA CALL FOR PRICEAdd To Favorites	Add To Favorites
45-254M-15	0.5 mL	$0.00 1/EA CALL FOR PRICEAdd To Favorites	Add To Favorites
45-254M-16	1 mL	$0.00 1/EA CALL FOR PRICEAdd To Favorites	Add To Favorites
45-254M-17	1 mL	$0.00 1/EA CALL FOR PRICEAdd To Favorites	Add To Favorites
45-254M-18	7 mL	$0.00 1/EA CALL FOR PRICEAdd To Favorites	Add To Favorites

Immunohistochemistry FLI-1 (MRQ-1) on ewing sarcoma

antibody form	culture supernatant
antibody product type	primary antibodies
biological source	mouse
clone	MRQ-1, monoclonal
conjugate	unconjugated
control	pnet
description	For In Vitro Diagnostic Use in Select Regions (See Chart)
form	buffered aqueous solution
isotype	IgG2b
manufacturer/tradename	Cell Marque^™
packaging	bottle of 1.0 mL predilute (254M-17)
	bottle of 7.0 mL predilute (254M-18)
	vial of 0.1 mL concentrate (254M-14)
	vial of 0.5 mL concentrate (254M-15)
	vial of 1.0 mL concentrate (254M-16)
Quality Level	100
	500
shipped in	wet ice
species reactivity	human
storage temp.	2-8°C
technique(s)	immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:25-1:100
visualization	nuclear

General description:

Ewing sarcoma/peripheral primitive neuroectodermal tumor (ES/PNET) is a rare primary tumor of the bone/soft tissue that resembles other undifferentiated tumors. The differential diagnosis of undifferentiated tumors of the soft tissue includes blastemal Wilms tumor, rhabdoid tumor, neuroblastoma, lymphoma, clear cell sarcoma, small cell carcinoma, synovial sarcoma (SS), neuroblastoma, desmoplastic small round cell tumor (DSRCT), and ES/PNET. It is important to correctly classify these tumors for appropriate treatment. The most common primitive renal tumor, Wilms tumor, responds well to a standard regimen of multiagent chemotherapy, whereas renal ES/PNET tends to be a high-stage, aggressive neoplasm that requires more extensive therapy .

The FLI-1 gene and FLI-1 protein are best known for their critical role in the pathogenesis of ES/PNET. More than 85% of ES/PNET are characterized by the translocation t(11;22)(q24;q12) that results in the fusion of the ews gene on chromosome 22 to the FLI-1 gene on chromosome 11. FLI-1 is a member of the ETS (erythroblastosis virus-associated transforming sequences) family of DNA-binding transcription factors and is involved in cellular proliferation and tumorigenesis. FLI-1 is normally expressed in endothelial cells and in hematopoietic cells, including T lymphocytes. The immunohistochemical detection of FLI-1 protein has been shown in two recent studies to be valuable in the discrimination of ES/PNET from most of its potential mimics, with the notable exception of lymphoblastic lymphoma.

The FLI-1 gene has also recently been shown to play an important role in the embryologic development of blood vessels. Expression of FLI-1 protein in adult endothelial cells in all types of blood vessels (arterial, venous, and lymphatic) has previously been shown both in our previous work and in that of Nilsson et al.

Folpe et al. found FLI-1 to be a highly sensitive (92%) and, with regards to the cases evaluated in this study, specific (100%) marker of both benign and malignant vascular tumors. The “absolute specificity” of FLI-1 is of course lower, given its expression in ES/PNET and lymphomas. FLI-1 expression appears to be the first reliable nuclear marker of endothelial differentiation. In particular, Folpe et al. found that FLI-1 reliably distinguished epithelioid forms of angiosarcoma from two important mimics, epithelioid sarcoma and carcinoma.

Assoc. products: WT-1, CD99, Synaptophysin, Chromogranin A, CK AE1/AE3

Legal Information:

Cell Marque is a trademark of Merck KGaA, Darmstadt, Germany

Linkage:

FLI-1 Positive Control Slides, Product No. 254S External link

, are available for immunohistochemistry (formalin-fixed, paraffin-embedded sections).

Other Notes:

For Technical Service please contact: 800-665-7284 or email: service@cellmarque.com

Physical form:

Solution in Tris Buffer, pH 7.3-7.7, with 1% BSA and <0.1% Sodium Azide

Preparation Note:

Download the IFU specific to your product lot and format External link

Note: This requires a keycode which can be found on your packaging or product label.

Quality:

IVD

RUO

RIDADR	NONH for all modes of transport
WGK Germany	WGK 2
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Storage Temp.	2-8°C
UNSPSC	12352203