GCDFP-15 (EP1582Y) Rabbit Monoclonal Primary Antibody

SIGMA/257R-1

Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-257R-14	0.1 mL	$0.00 1/EA CALL FOR PRICEAdd To Favorites	Add To Favorites
45-257R-15	0.5 mL	$0.00 1/EA CALL FOR PRICEAdd To Favorites	Add To Favorites
45-257R-16	1 mL	$0.00 1/EA CALL FOR PRICEAdd To Favorites	Add To Favorites
45-257R-17	1 mL	$0.00 1/EA CALL FOR PRICEAdd To Favorites	Add To Favorites
45-257R-18	7 mL	$0.00 1/EA CALL FOR PRICEAdd To Favorites	Add To Favorites

Immunohistochemistry GCDFP-15 (EP1582Y) on breast carcinoma

antibody form	culture supernatant
antibody product type	primary antibodies
biological source	rabbit
clone	EP1582Y, monoclonal
conjugate	unconjugated
control	breast, breast carcinoma
description	For In Vitro Diagnostic Use in Select Regions (See Chart)
form	buffered aqueous solution
isotype	IgG
manufacturer/tradename	Cell Marque^™
packaging	bottle of 1.0 mL predilute (257R-17)
	bottle of 7.0 mL predilute (257R-18)
	vial of 0.1 mL concentrate (257R-14)
	vial of 0.5 mL concentrate (257R-15)
	vial of 1.0 mL concentrate (257R-16)
Quality Level	100
	500
shipped in	wet ice
species reactivity	human
storage temp.	2-8°C
technique(s)	immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100-1:500
visualization	cytoplasmic

General description:	Gross cystic disease fluid protein-15 (GCDFP-15) is one of four major component proteins found in the cystic fluid obtained from patients with fibrocystic changes of the breast. GCDFP-15 is a marker of apocrine glandular differentiation in both benign and malignant mammary epithelium. This protein has a widespread distribution in apocrine glands elsewhere in the axillary and perianal tissues, as well as in the sublingual and submaxillary salivary glands. The GCDFP-15 protein is a 15 kDa glycoprotein shown to be prolactin-inducible, and the GCDFP-15 gene has been cloned. Ultrastructurally, the GCDFP-15 protein has been localized in Golgi vesicle and cytoplasmic granules. The protein is released by exocytosis at the apices of the mammary epithelial cells. The primary application for this antibody product is the identification of breast carcinoma in the metastatic setting, or fluid analysis. In a study by Wick et al. on paraffin sections of 105 breast cancers and 585 non-mammary malignancies in order to assess its value in this context, anti-GCDFP-15 was compared with another putative mammary epithelial marker, alpha-lactalbumin (ALA), with respect to sensitivity and specificity for a diagnosis of breast carcinoma. Overall, the rates of specificity and sensitivity and positive predictive value for GCDFP-15 were 95%, 74%, and 74%, respectively. A consistent congruency between the reactivity patterns of primary and metastatic breast cancers was noted for GCDFP-15. Besides mammary carcinomas, the major tumor types that express GCDFP-15 were carcinomas of the salivary glands and sweat glands. Since the latter two types of lesions are unlikely to be diagnosed as metastatic breast cancer, statistical indices were recalculated after exclusion of these three tumor types. Following this exclusion, the adjusted rate of specificity of GCDFP-15 and positive predictive value for a diagnosis of metastatic carcinoma of the breast were each 99%. Another study by Bhargava et al. has shown GCDFP-15 staining of a higher percentage of cancer cells in lobular carcinoma as compared to ductal carcinoma of the breast.
Legal Information:	Cell Marque is a trademark of Merck KGaA, Darmstadt, Germany
Linkage:	GCDFP-15 Positive Control Slides, Product No. 257S , are available for immunohistochemistry (formalin-fixed, paraffin-embedded sections).
Other Notes:	For Technical Service please contact: 800-665-7284 or email: service@cellmarque.com
Physical form:	Solution in Tris Buffer, pH 7.3-7.7, with 1% BSA and <0.1% Sodium Azide
Preparation Note:	Download the IFU specific to your product lot and format Note: This requires a keycode which can be found on your packaging or product label.

RIDADR	NONH for all modes of transport
WGK Germany	WGK 2
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Storage Temp.	2-8°C
UNSPSC	12352203