Brilliant Blue G

SIGMA/27815 - 250, for microscopy

Synonym: Acid blue 90; Coomassie Brilliant Blue G

CAS Number: 6104-58-1
Empirical Formula (Hill Notation): C₄₇H₄₈N₃NaO₇S₂
Molecular Weight: 854.02
EC Number: 228-058-4
MDL Number: MFCD00078482
Linear Formula: C₄₇H₄₈N₃NaO₇S₂
Product Type: Chemical

Catalog Number	PKG Qty.	Price	Quantity


45-27815-25G-F	25 g	$61.90 1/EAAdd To Favorites		Add To Favorites
45-27815-100G-F	100 g	$190.00 1/EAAdd To Favorites		Add To Favorites

form	solid
grade	for microscopy
InChI	1S/C47H49N3O7S2.Na/c1-6-49(31-35-11-9-13-43(29-35)58(51,52)53)40-21-25-45(33(4)27-40)47(37-15-17-38(18-16-37)48-39-19-23-42(24-20-39)57-8-3)46-26-22-41(28-34(46)5)50(7-2)32-36-12-10-14-44(30-36)59(54,55)56;/h9-30H,6-8,31-32H2,1-5H3,(H2,51,52,53,54,55,56);/q;+1/p-1
InChI key	RWVGQQGBQSJDQV-UHFFFAOYSA-M
Quality Level	200
SMILES string	[Na+].CCOc1ccc(Nc2ccc(cc2)C(c3ccc(cc3C)N(CC)Cc4cccc(c4)S([O-])(=O)=O)=C5C=CC(C=C5C)=[N+](CC)Cc6cccc(c6)S([O-])(=O)=O)cc1
technique(s)	microbe id \| staining: suitable

Analysis Note:	λ_max. ∼610 nm; E(1%,1 cm) ∼500-700 (H₂O)
Application:	Brilliant Blue G was used to prepare the protein reagent for the determination of protein content of the collagenase enzyme isolated from fish waste. It may be employed as stain for the internal limiting membrane (ILM) for the macular hole (MH) and epiretinal membrane (ERM) surgery.
Biochem/physiol Actions:	P2X7 purinergic receptor antagonist.
General description:	Brilliant Blue G (BBG) is a dye. It has been proposed as a substitute for internal limiting membrane (ILM) peeling.
Other Notes:	Protein stain for gel electrophoresis. Does not penetrate the gel and is three times more sensitive than Naphthol Blue Black B; Simple and sensitive protein assay; Rapid, sensitive and versatile assay for proteins; free amino acids and several chemicals that interfere with the Lowry procedure cause no interference.