Atto 425-Biotin

SIGMA/28616 - BioReagent, suitable for fluorescence, ≥95.0% (HPCE)

Synonym: Biotin-Atto 425

Empirical Formula (Hill Notation): C₃₇H₅₃N₅O₇S
Molecular Weight: 711.91
MDL Number: MFCD04974818
Linear Formula: C₃₇H₅₃N₅O₇S
Product Type: Chemical

Catalog Number	PKG Qty.	Price	Quantity


45-28616-1MG-F	1 mg	$1180.00 1/EAAdd To Favorites		Add To Favorites

assay	≥95.0% (HPCE)
fluorescence	λ_ex 437 nm; λ_em 483 nm in 0.1 M phosphate pH 7.0
form	powder
InChI	1S/C37H53N5O7S/c1-5-48-34(45)26-19-24-18-25-23(2)21-37(3,4)42(28(25)20-29(24)49-35(26)46)17-11-14-32(44)39-16-10-6-9-15-38-31(43)13-8-7-12-30-33-27(22-50-30)40-36(47)41-33/h18-20,23,27,30,33H,5-17,21-22H2,1-4H3,(H,38,43)(H,39,44)(H2,40,41,47)
InChI key	LPVYZZMUDCZDQO-UHFFFAOYSA-N
manufacturer/tradename	ATTO-TEC GmbH
product line	BioReagent
Quality Level	100
SMILES string	CCOC(=O)C1=Cc2cc3C(C)CC(C)(C)N(CCCC(=O)NCCCCCNC(=O)CCCCC4SCC5NC(=O)NC45)c3cc2OC1=O
storage temp.	−20°C
suitability	suitable for fluorescence

Application:	Atto fluorescent labels are designed for high sensitivity applications, including single molecule detection. Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation. Atto 425 biotin is suitable for fluorescence labeling of avidin/streptavidin conjugated molecules.
General description:	Atto 425 is a new label with high quantum yield (0.90) as well as sufficient stoke′s shift (excitation maximum 436 nm, emission maximum 484 nm). It is optimized for excitation with argon laser, and is characterized by high photostability. It does nearly not show cis-trans-isomerization, which limits brightness and reproducibility for many other dyes.
Legal Information:	This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.