MUC1 (EP85) Rabbit Monoclonal Primary Antibody
SIGMA/290R-1
Synonym: Mucin 1
Product Type: Chemical
Immunohistochemistry MUC1 (EP85) on invasive ductal carcinoma
| antibody form | culture supernatant |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | EP85, monoclonal |
| conjugate | unconjugated |
| control | breast, colon (and Associated adenocarcinomas) |
| description | For In Vitro Diagnostic Use in Select Regions |
| form | buffered aqueous solution |
| isotype | IgG |
| manufacturer/tradename | Cell Marque™ |
| packaging | vial of 0.1 mL concentrate (290R-14) |
| vial of 0.1 mL concentrate Research Use Only (290R-14-RUO) | |
| vial of 0.5 mL concentrate (290R-15) | |
| vial of 1.0 mL concentrate (290R-16) | |
| vial of 1.0 mL concentrate Research Use Only (290R-16-RUO) | |
| vial of 1.0 mL pre-dilute ready-to-use (290R-17) | |
| vial of 1.0 mL pre-dilute Research Use Only (290R-17-RUO) | |
| vial of 7.0 mL pre-dilute ready-to-use (290R-18) | |
| vial of 7.0 mL pre-dilute ready-to-use Research Use Only (290R-18-RUO) | |
| Quality Level | 100  |
| 500 |
|
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | 2-8°C |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100-1:500 (concentrated) |
| visualization | cytoplasmic |
| General description: | Mucin 1 (MUC1) is a transmembrane glycoprotein that protects epithelial cells from injury caused by external stimuli. In addition to this role, MUC1 is involved in cell-cell adhesion, proliferation, motility, invasion and survival. In epithelial cells, MUC1 expression is regulated by binding of TNFα to TNFR1 and activation of the NFαB pathway. In human skin, MUC1 is not expressed in normal epidermis but rather in pre-malignant and malignant conditions. MUC1 has also been expressed in the epidermis of psoriatic plaques of biopsies from patients diagnosed with psoriasis vulgaris. MUC1, in addition to being localized at the apical surface of some suprabasal keratinocytes, is also localized over the entire cell surface of some of these cells and some basal keratinocytes. Conversely, no MUC1 immunoreactivity is typically detected in the epidermis of normal skin. Reactive gastropathy is the second most common diagnosis made on gastric biopsies. Increased epithelial proliferation and modifications of epithelial cytokeratin profile, distinct from those of Helicobacter pylori gastritis, have been previously reported. Loss of MUC1, either patchy or complete, was noted in 67% of the cases. The overexpression of MUC1 was found in 78% of papillary thyroid carcinomas (PTC). Multivariate analysis has shown a strong association of MUC1 expression with the presence of the BRAF (V600E) mutation and lymph node metastasis in PTC. Lymph node metastasis was the most important risk factor of relapse. In a study of thymic epithelial tumors, MUC1 was closely associated with VEGF, p53 and micro-vessel density (MVD). A 94% positive rate of MUC1 expression in thymic carcinomas and 0% in type B3 thymomas, demonstrated a significant difference. The expression of MUC1 was closely related to the grade of malignancy in thymic epithelial tumors. Positive expression of MUC1 was significantly associated with poor outcome in this disease. MUC1 may be useful marker for differentiating thymic carcinoma from type B3 thymoma.5 | ||||
| Legal Information: | Cell Marque is a trademark of Merck KGaA, Darmstadt, Germany | ||||
| Linkage: | MUC1 Positive Control Slides, Product No. 290S , are available for immunohistochemistry (formalin-fixed, paraffin-embedded sections). |
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| Other Notes: | For Technical Service please contact: 800-665-7284 or email: service@cellmarque.com | ||||
| Physical form: | Solution in Tris Buffer, pH 7.3-7.7, with 1% BSA and <0.1% Sodium Azide. | ||||
| Preparation Note: | Download the IFU specific to your product lot and format  Note: This requires a keycode which can be found on your packaging or product label. Download the latest released IFU Note: This IFU may not apply to your specific product lot. |
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| Quality: |
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| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |