BOB.1 (SP92) Rabbit Monoclonal Antibody
SIGMA/294R-1
Product Type: Chemical
Immunohistochemistry BOB.1 (SP92) on tonsil
| antibody form | culture supernatant |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | SP92, monoclonal |
| conjugate | unconjugated |
| control | B-cell lymphoma, tonsil |
| description | For In Vitro Diagnostic Use in Select Regions (See Chart) |
| form | buffered aqueous solution |
| isotype | IgG1 |
| manufacturer/tradename | Cell Marque® |
| packaging | bottle of 1.0 mL predilute (294R-17) |
| bottle of 7.0 mL predilute (294R-18) | |
| vial of 0.1 mL concentrate (294R-14) | |
| vial of 0.5 mL concentrate (294R-15) | |
| vial of 1.0 mL concentrate (294R-16) | |
| Quality Level | 100  |
| 500 |
|
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | 2-8°C |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100-1:500 |
| visualization | nuclear |
| General description: | Expression of BOB.1/OBF.1 is restricted largely to mature B-cells. Germinal center B-cells normally demonstrate strong staining for BOB.1, as do mantle-zone B-cells, and plasma cells. Analyses of BOB.1/OBF.1 expression in a variety of established B-cell lines representing different stages of B-cell development has suggested a constitutive, B-cell-specific expression pattern. Because they are germinal center derived, L&H cells in nodular lymphocyte predominant Hodgkin lymphoma are consistenty immunoreactive for BOB.1. Conversely, the Hodgkin/Reed- Sternberg cells in classical Hodgkin lymphoma either do not express both (80%) or express only one (20%) of the two proteins. In B-cell lymphomas, the highest expression levels for BOB.1/OBF.1 are reported in follicular center lymphomas, diffuse large B-cell lymphomas and Burkitt lymphomas.B-CLL, MALT-type, and mantle cell lymphomas score negative or display an heterogenous/weaker reactivity.10-11 Studies have suggested that anti-BOB. has a high predictive value in discriminating primary mediastinal B-cell lymphoma from classical Hodgkin disease.Approximately 50% of acute myeloid leukemias express BOB.1. |
| Legal Information: | Cell Marque is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Other Notes: | BOB.1 Positive Control Slides, Product No. 294S , are available for immunohistochemistry (formalin-fixed, paraffin-embedded sections). |
| Other Notes: | For Technical Service please contact: 800-665-7284 or email: service@cellmarque.com |
| Physical form: | Solution in Tris Buffer, pH 7.3-7.7, with 1% BSA and <0.1% Sodium Azide |
| Preparation Note: | Download the IFU specific to your product lot and format  Note: This requires a keycode which can be found on your packaging or product label. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |