Human Brachiocephalic Artery Endothelial Cells: HBcAEC, adult

SIGMA/3012-05A

Product Type: Product-on-demand

Catalog Number	PKG Qty.	Price	Quantity


45-3012-05A	1 each	$0.00 1/EA CALL FOR PRICEAdd To Favorites		Add To Favorites

Cell Applications : Left: Human Brachiocephalic Artery Endothelial Cells, HBcAEC Right: HBcAEC immunolabeled for vWF (green); nuclei are counterstained with PI (red).

biological source	human brachiocephalic artery (normal)
growth mode	Adherent
karyotype	2n = 46
manufacturer/tradename	Cell Applications, Inc
morphology	Endothelial
packaging	pkg of 500,000 cells
Quality Level	100
relevant disease(s)	cardiovascular diseases
shipped in	dry ice
storage temp.	−196°C
technique(s)	cell culture \| mammalian: suitable

Application:	oxygenated blood supply, endothelial permeability, blood albumin leakage, endothelial integrity
Cell Line Origin:	Artery
Components:	Basal Medium containing 10% FBS & 10% DMSO
Disclaimer:	RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
General description:	Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details. HBcAEC, along with human aortic (HAOEC), carotid artery (HCtAEC), coronary artery (HCAEC) and subclavian artery (HScAEC) have been used to demonstrate that not only blood vessels from different tissues are highly heterogeneous, they also interact differently with leukocytes during the inflammation response (Scott, 2013). The authors further showed that differential N-glycosylation of commonly expressed vascular adhesion molecules may be responsible for this heterogeneity, as well as for modulation of signaling under resting and activated inflammatory conditions. This also explains why specific vascular beds may be more or less susceptible to particular diseases or stimuli. Importantly, if cells from different sources were used, these results could not be convincingly validated due to a number of uncontrolled variables, such as age, race, genetic variability or life style choices of the donors. Because of the complex heterogeneity that exists not only between different donors, but even between different vascular beds in the same individual, it would be prudent to confirm any new findings on primary cell lots coming from several different origins.
Preparation Note:	• 2nd passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO • Can be cultured at least 12 doublings
Subculture Routine:	Please refer to the HBcAEC Culture Protocol .