Product Type: Chemical
| shipped in | dry ice |
| storage temp. | −20°C |
| General description: | The Transcreener® EPIGEN Methyltransferase Assay is a universal biochemical HTS assay for enzymes that produce S-adenosylhomocysteine (SAH), including all enzymes within the histone (HMTs) and DNA (DNMTs) methyltransferase families. It combines the extensively validated Transcreener® AMP2/GMP2 Assay, which relies on fluorescent immunodetection of AMP, with coupling enzymes that convert SAH to AMP. Enzyme activity is signaled by a decrease in fluorescence polarization as the bound tracer is displaced from the Transcreener® AMP2/GMP2 Antibody. The assay is a simple mix-and-read format with two liquid addition steps. Methyltransferase (MT) enzyme reactions are first quenched with Stop Buffer and then the SAH Detection Mixture containing coupling enzymes, Transcreener® AMP2/GMP2 antibody, and tracer is added. The assay provides excellent signal at low substrate conversion, with a Z’ =0.7 and =100 millipolarization shift (mP) under normal reaction conditions. View full Transcreener® product list  |
| Legal Information: | Transcreener is a registered trademark of BellBrook Labs |
| Physical form: | Kit with buffered aqueous solutions |
| Quantity: | 3017-1K = 1,000 assay, 384-well 3017-10K = 10,000 assay, 384-well |
| Storage Temp. | −20°C |
| UNSPSC | 41122100 |
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