Transcreener^® GDP TR-FRET Assay

SIGMA/3021

Product Type: Chemical

Catalog Number	PKG Qty.	Price	Quantity


45-3021-10K	1 each	$0.00 1/EA CALL FOR PRICEAdd To Favorites		Add To Favorites
45-3021-1K	1 each	$0.00 1/EA CALL FOR PRICEAdd To Favorites		Add To Favorites

shipped in	dry ice
storage temp.	−20°C

General description:	The Transcreener^® GDP TR-FRET Red Assay is a competitive immunoassay for GDP with a far-red, time-resolved Förster-resonance-energy-transfer (TR-FRET) readout. Because it is highly selective for GDP, the assay can be used with any enzyme that converts GTP to GDP, regardless of what other substrates are used. Examples of enzymes include GTPases and fucosyltransferases. The Transcreener^® assay is designed specifically for high-throughput screening (HTS), with a single-addition, mix-and-read format. It offers reagent stability and compatibility with commonly used multimode plate readers. The generic nature of the Transcreener^® HTS assay platform eliminates delays involved in assay development for new HTS targets and greatly simplifies compound and inhibitor profiling across multiple target families. The Transcreener^® GDP TR-FRET Red Assay provides the following benefits: • Accommodates GTP concentrations ranging from 0.1 μM to 1,000 μM. • Excellent data quality (Z’ = 0.7) at low substrate conversion (typically 10–30%). • Overcomes the need for time-consuming, one-off assay development for individual members within a group transfer enzyme family by using a single set of assay reagents that detect an invariant product. • Time-gated detection method largely eliminates interference that can result from prompt fluorescence of test compounds. • Far-red tracer further minimizes interference from fluorescent compounds and light scattering. View full Transcreener^® product list
Legal Information:	Transcreener is a registered trademark of BellBrook Labs
Physical form:	Kit with buffered aqueous solutions
Quantity:	3021-1K = 1,000 assay, 384-well 3021-10K = 10,000 assay, 384-well