Nerve Growth Factor Receptor (NGFR) (MRQ-21) Mouse Monoclonal Antibody

SIGMA/304M-1

Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-304M-14	0.1 mL	$0.00 1/EA CALL FOR PRICEAdd To Favorites	Add To Favorites
45-304M-15	0.5 mL	$0.00 1/EA CALL FOR PRICEAdd To Favorites	Add To Favorites
45-304M-16	1 mL	$0.00 1/EA CALL FOR PRICEAdd To Favorites	Add To Favorites
45-304M-17	1 mL	$0.00 1/EA CALL FOR PRICEAdd To Favorites	Add To Favorites
45-304M-18	7 mL	$0.00 1/EA CALL FOR PRICEAdd To Favorites	Add To Favorites

Immunohistochemistry Nerve Growth Factor Receptor (NGFR) on breast (sclerosing adenosis)

antibody form	diluted ascites fluid
antibody product type	primary antibodies
biological source	mouse
clone	MRQ-21, monoclonal
conjugate	unconjugated
control	breast
description	For In Vitro Diagnostic Use in Select Regions (See Chart)
form	buffered aqueous solution
isotype	IgG1
manufacturer/tradename	Cell Marque^™
packaging	bottle of 1.0 mL predilute (304M-17)
	bottle of 7.0 mL predilute (304M-18)
	vial of 0.1 mL concentrate (304M-14)
	vial of 0.5 mL concentrate (304M-15)
	vial of 1.0 mL concentrate (304M-16)
Quality Level	100
	500
shipped in	wet ice
species reactivity	human
storage temp.	2-8°C
technique(s)	immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100-1:500
visualization	cytoplasmic

General description:

NGFR, a 75 kd glycoprotein (also known as P-75NTR), is the first of neurotrophin receptors to be isolated and is a member of the tumor necrosis factor (TNF) receptor family. It is expressed not only in sympathetic and sensory neurons, but also in various neural crest cell or tumor derivatives such as melanocytes, melanomas, neuroblastomas, pheochromocytomas, neurofibromas, and neurotized nevi (type C melanocytes). NGFR has been shown to be a reliable marker for desmoplastic and neurotropic melanoma by several groups. Although the sensitivity of NGFR staining in desmoplastic melanomas has not been systematically analyzed, all reported cases stain with NGFR. A review of 9 cases of desmoplastic melanomas diagnosed in the Dermatopathology Section of the Department of Dermatology at Boston University School of Medicine between 2001 and 2004 disclosed that all 9 cases of desmoplastic melanomas tested stained positively with NGFR. These findings are in contrast to a study reported by Huttenbach et al in which only 33% of their desmoplastic melanomas stained with NGFR. This difference emphasizes the importance of the choice of monoclonal antibody to obtain consistent results. This staining property of desmoplastic melanoma cells can be useful in diagnosing challenging cases, namely early lesions, suspected cases with negative or weak S-100 staining, and those that must be distinguished from a scar. It is now apparent that expression of NGFR is ubiquitous and not limited to the nervous system, being expressed in mature nonneural cells such as perivascular cells, dental pulp cells, lymphoidal follicular dendritic cells, basal epithelium of oral mucosa and hair follicles, prostate basal cells and myoepithelial cells. Unlike the high-affinity nerve growth factor tyrosine kinase receptors (TrkA, TrkB and TrkC), NGFR has no intrinsic tyrosine kinase activity. Studies in prostate and urothelial cancer suggest that NGFR may act as a tumour suppressor, negatively regulating cell growth and proliferation. Anti-NGFR labels the myoepithelial cells of breast ducts and intralobular fibroblasts of breast ducts and thus aides in the diagnosis of malignancy in the breast. Associated products: GFAP, S.M. Myosin, Calponin, NSE, Myelin Basic Protein, S-100, HMB45, Tyrosinase, MART-1

Legal Information:

Cell Marque is a trademark of Merck KGaA, Darmstadt, Germany

Linkage:

Nerve Growth Factor Receptor (NGFR) Positive Control Slides, Product No. 304S External link

, are available for immunohistochemistry (formalin-fixed, paraffin-embedded sections).

Other Notes:

For Technical Service please contact: 800-665-7284 or email: service@cellmarque.com

Physical form:

Solution in Tris Buffer, pH 7.3-7.7, with 1% BSA and <0.1% Sodium Azide

Preparation Note:

Download the IFU specific to your product lot and format External link

Note: This requires a keycode which can be found on your packaging or product label.

Quality:

IVD

RUO

Storage Temp.	2-8°C
UNSPSC	12352203