Human Cardiac Fibroblasts: HCF, fetal

SIGMA/306-05F

Synonym: Cardiac Fibroblasts; Fetal Cardiac Fibroblasts; HCF Cardiac Fibroblasts

Product Type: Product-on-demand

Catalog Number	PKG Qty.	Price	Quantity


45-306-05F	1 each	$908.00 1/EAAdd To Favorites		Add To Favorites

Cell Applications : Human Cardiac Fibroblasts, HCF (left). HCF immunolabeled for FSP (right, green). Nuclei are stained with PI.

biological source	human heart (normal)
growth mode	Adherent
karyotype	2n = 46
manufacturer/tradename	Cell Applications, Inc
morphology	fibroblast
packaging	pkg of 500,000 cells
Quality Level	100
relevant disease(s)	cardiovascular diseases
shipped in	dry ice
storage temp.	−196°C
technique(s)	cell culture \| mammalian: suitable

Application:	human heart function, neointimal formation, vascular remodeling, myocardial repair, extracellular maintenance, signaling, toxicology, gene expression, structural support for cardiomycytes, synthesis of extracellular matrix, growth factors and cytokines
Cell Line Origin:	Heart
Components:	Basal Medium containing 10% FBS & 10% DMSO
Disclaimer:	RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
General description:	Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details. Cardiac fibroblasts are the most prevalent cell type in the heart, making up 60-70 % of all cells. HCF from Cell Applications, Inc. provide an excellent model system to study many aspects of human heart function and pathophysiology. HCF has been utilized in a number of research publications, for example to: • Determine that electrical coupling between cardiomyocytes and fibroblasts is mediated by large-conductance Ca2+-activated K+ channels that can be stimulated by estrogen receptor agonists (Wang, 2006, 2007); and show that antimitogenic effects of estradiol on HCF growth are mediated by cytochromes 1A1/1B1-and catechol-O-methyltransferase-derived metabolites (Dubey, 2005) • Show that in response to mechanical stretch, cardiac fibroblasts release TGF-β which causes trombomodulin downregulation, increasing the risk of thromboembolic events (Kapur, 2007) and also induces cardiac fibroblast differentiation into myofibroblasts via increased Smad2 and ERK1/2 phosphorylation, that could be stimulated by endothelin-1 and inhibited by Ac-SDKP (Peng, 2010) • Demonstrate that activation of G protein-coupled receptor kinase-2 (GRK2) prevents normal regulation of collagen synthesis in cardiac fibroblasts mimicking heart failure phenotype (D’Souza, 2011); identify FGF2 signaling pathway as potential target for modulating apoptosis in cardiac pathology (Ma, 2011) and investigate the roles of scleraxis (Bagchi, 2012) and AMPKα1 (Noppe, 2014) in scar formation following myocardial infarction • Show that the KATP channel opener KMUP-3 preserved cardiac function after myocardial infarction by enhancing the expression of NO synthase and restoring MMP-9/TIMP-1 balance (Liu, 2011) HCF were also shown to express delayed rectifier IK, Ito, Ca2+-activated K+ current (BKCa), inward-rectifier (Kir-type), and swelling-induced Cl- current (ICl.vol) channels (Yue, 2013).
Preparation Note:	• 1st passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO • Can be cultured at least 8 doublings
Subculture Routine:	Please refer to the HCF Culture Protocol .