Chromeo™ P503
SIGMA/30693
Product Type: Chemical
fluorescence | λex 503 nm; λem 627 nm±10 nm in 0.1 M bicarbonate buffer pH 8.3 |
Quality Level | 100 ![]() |
storage temp. | −20°C |
Application: | Chromeo P503 is used as a fluorogenic reagent to label primary amine groups within molecules such as proteins. Chromeo P503 labeled proteins may be studied in a variety of gel electrophoresis and chromatographic applications. |
Caution: | To ensure stability, the lyophilized dye should be stored at 4°C in the dark. This product is guaranteed for 6 months from the date of arrival. |
General description: | Chromeo P503 labels proteins and peptides by exhibiting a color change from blue to red upon binding to primary amines. Chromeo P503 displays a weak fluorescence with a quantum yield <1% in solution. After conjugation to a primary amine group, the label undergoes a shortwave spectral shift of >100 nm and the quantum yield rises to 50%. This property allows a distinct detection of primary amines, proteins, and other biomolecules. |
Legal Information: | Chromeo is a trademark of Active Motif Chromeon GmbH |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 3 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Storage Temp. | −20°C |
UNSPSC | 12352108 |