Human Internal Thoracic Artery Endothelial Cells: HITAEC, adult

SIGMA/308-05A

Product Type: Product-on-demand

Catalog Number	PKG Qty.	Price	Quantity


45-308-05A	1 each	$0.00 1/EA CALL FOR PRICEAdd To Favorites		Add To Favorites

Cell Applications : HITAEC immunolabeled for vWF (middle, green) and CD31/PECAM (right, green). Nuclei are visualized with PI (red).

biological source	human intrathoratic artery (normal)
growth mode	Adherent
karyotype	2n = 46
manufacturer/tradename	Cell Applications, Inc
morphology	Endothelial
packaging	pkg of 500,000 cells
Quality Level	100
relevant disease(s)	diabetes; cardiovascular diseases
shipped in	dry ice
storage temp.	−196°C
technique(s)	cell culture \| mammalian: suitable

Application:	coronary artery bypass grafting, myocardial revascularization, endothelial cell motility, transendothelial migration, gene expression, contractile responses, receptor stimulation, cell relaxation, supply of oxygenated blood to chest wall and breasts,
Cell Line Origin:	Artery
Components:	Basal Medium containing 10% FBS & 10% DMSO
Disclaimer:	RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
General description:	Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details. HITAEC has been used to investigate oral pathogens travel through the body by infecting dendritic cells and inducing them to migrate through endothelial layers by causing upregulation of CXCR4 (Miles, 2014). To identify genes that make Human Internal Thoracic Arteries resistant to atherosclerosis, HITAEC and HCAEC (derived from the athero-prone human coronary arteries) were subjected to microarray analysis (Archaki, 2012). Twenty-nine genes were found to be expressed differently between HITAEC and HCAEC; one of them (TES) was shown to be downregulated in patients with coronary artery disease, resulting in increased monocyte adhesion, endothelial cell motility and transendothelial migration of monocytes.
Preparation Note:	• 2nd passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO • Can be cultured at least 10 doublings
Subculture Routine:	Please refer to the HITAEC Culture Protocol .