Oct-4 (MRQ-10) Mouse Monoclonal Antibody
SIGMA/309M-1
Product Type: Chemical
Immunohistochemistry Oct-4 (MRQ-10) on seminoma
| antibody form | diluted ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | MRQ-10, monoclonal |
| conjugate | unconjugated |
| control | seminoma |
| description | For In Vitro Diagnostic Use in Select Regions (See Chart) |
| form | buffered aqueous solution |
| isotype | IgG |
| manufacturer/tradename | Cell Marque™ |
| packaging | bottle of 1.0 mL predilute (309M-17) |
| bottle of 7.0 mL predilute (309M-18) | |
| vial of 0.1 mL concentrate (309M-14) | |
| vial of 0.5 mL concentrate (309M-15) | |
| vial of 1.0 mL concentrate (309M-16) | |
| Quality Level | 100  |
| 500 |
|
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | 2-8°C |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50-1:200 |
| visualization | nuclear |
| General description: | Oct-4 is a transcription factor that functions in the regulation and maintenance of pluripotency in embryonic stem and primordial germ cells. Oct-4 immunoreactivity has been demonstrated in gonadal and extra-gonadal seminomas, dysgerminomas, and embryonal carcinomas. In addition, the immunohistochemical detection of Oct-4 assists in the evaluation of intratubular germ cell neoplasia (IGCN). | ||||
| General description: | Oct-4 is a transcription factor that maintains and regulates pluripotency in embryonic stem and germ cells. Anti-Oct-4 has shown a very high sensitivity and specificity in seminoma/dysgerminoma, embryonal carcinoma, and the germ cell component of gonadoblastoma by nuclear immunostaining. Clear cell carcinoma may enter the differential diagnosis of dysgerminoma as both may grow in nests or tubules, contain clear cells, and have a prominent inflammatory infiltrate (lymphocytes in dysgerminoma and plasma cells in clear cell carcinoma). In one study that looked at anti-Oct-4 staining in clear cell carcinomas, 4 of 14 tumors were found to be focally positive. In another study, 49 endometrioid carcinomas were Oct-4 negative. Rarely dysgerminoma may have a morphologic appearance that overlaps with sex cord-stromal tumors, especially poorly differentiated Sertoli cell tumors. In two studies however, all sex cord-stromal tumors of the testis and granulosa cell tumors of the ovary were Oct-4 negative. | ||||
| Legal Information: | Cell Marque is a trademark of Merck KGaA, Darmstadt, Germany | ||||
| Linkage: | Oct-4 Positive Control Slides, Product No. 309S , are available for immunohistochemistry (formalin-fixed, paraffin-embedded sections). |
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| Other Notes: | For Technical Service please contact: 800-665-7284 or email: service@cellmarque.com | ||||
| Physical form: | Solution in Tris Buffer, pH 7.3-7.7, with 1% BSA and <0.1% Sodium Azide | ||||
| Preparation Note: | Download the IFU specific to your product lot and format  Note: This requires a keycode which can be found on your packaging or product label. |
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| Quality: |
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| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352200 |