4′,6-Diamidino-2-phenylindole dihydrochloride
SIGMA/32670 - BioReagent, suitable for fluorescence, ≥95.0% (HPLC)
Synonym: 2-
CAS Number: 28718-90-3
Empirical Formula (Hill Notation): C16H15N5 · 2HCl
Molecular Weight: 350.25
EC Number: 249-186-7
MDL Number: MFCD00012681
Linear Formula: C16H15N5 · 2HCl
Product Type: Chemical
| assay | ≥95.0% (HPLC) |
| fluorescence | λex 340 nm; λem 488 nm (nur DAPI) |
| λex 364 nm; λem 454 nm (DAPI-DNA-Komplex) | |
| λex 374 nm; λem 461 nm in 10 mM Tris; 1 mM EDTA; pH 8.0; DNA | |
| form | powder |
| InChI | 1S/C16H15N5.2ClH/c17-15(1 |
| InChI key | FPNZBYLXNYPRLR-UHFFFAOYSA |
| product line | BioReagent |
| Quality Level | 100  |
| SMILES string | Cl.Cl.NC(=N)c1ccc(cc1)-c2 |
| solubility | DMF: soluble |
| H2O: soluble | |
| storage temp. | room temp |
| suitability | suitable for fluorescence |
| Application: | DAPI is several times more sensitive than ethidium bromide for staining DNA in agarose gels. It may be used for photofootprinting of DNA, to detect annealed probes in blotting applications by specifically visualizing the double-stranded complex, and to study the changes in DNA and analyze DNA content during apoptosis using flow cytometry. DAPI staining has also been shown to be a sensitive and specific detection method for mycoplasma. |
| Biochem/physiol Actions: | Cell permeable fluorescent minor groove-binding probe for DNA. Binds to the minor groove of double-stranded DNA (preferentially to AT rich DNA), forming a stable complex which fluoresces approximately 20 times greater than DAPI alone. |
| Packaging: | Bottomless glass bottle. Contents are inside inserted fused cone. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H315 - H317 - H335 |
| Precautionary statements | P261 - P264 - P271 - P272 - P280 - P302 + P352 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥95.0% (HPLC) |
| Storage Temp. | room temp |
| UNSPSC | 12171500 |