Advanced Search



4′,6-Diamidino-2-phenylindole dihydrochloride

SIGMA/32670 - suitable for fluorescence, BioReagent, ≥95.0% (HPLC)

Synonym: 2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride; DAPI dihydrochloride

CAS Number: 28718-90-3
Empirical Formula (Hill Notation): C16H15N5 · 2HCl
Molecular Weight: 350.25
EC Number: 249-186-7
MDL Number: MFCD00012681
Linear Formula: C16H15N5 · 2HCl
Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-32670-5MG-F 5 mg
 $126.00
1/EA		 Add To Favorites
45-32670-25MG-F 25 mg
 $519.00
1/EA		 Add To Favorites

 

assay ≥95.0% (HPLC)
fluorescence λex 340 nm; λem 488 nm (nur DAPI)
  λex 364 nm; λem 454 nm (DAPI-DNA-Komplex)
  λex 374 nm; λem 461 nm in 10 mM Tris; 1 mM EDTA; pH 8.0; DNA
form powder
InChI 1S/C16H15N5.2ClH/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;;/h1-8,21H,(H3,17,18)(H3,19,20);2*1H
InChI key FPNZBYLXNYPRLR-UHFFFAOYSA-N
product line BioReagent
Quality Level 100 
SMILES string Cl.Cl.NC(=N)c1ccc(cc1)-c2cc3ccc(cc3[nH]2)C(N)=N
solubility DMF: soluble
  H2O: soluble
storage temp. room temp
suitability suitable for fluorescence
Application: DAPI is several times more sensitive than ethidium bromide for staining DNA in agarose gels.
Application: DAPI is several times more sensitive than ethidium bromide for staining DNA in agarose gels. It may be used for photofootprinting of DNA, to detect annealed probes in blotting applications by specifically visualizing the double-stranded complex, and to study the changes in DNA and analyze DNA content during apoptosis using flow cytometry. DAPI staining has also been shown to be a sensitive and specific detection method for mycoplasma.
Biochem/physiol Actions: Cell permeable fluorescent minor groove-binding probe for DNA. Binds to the minor groove of double-stranded DNA (preferentially to AT rich DNA), forming a stable complex which fluoresces approximately 20 times greater than DAPI alone.
Packaging: Bottomless glass bottle. Contents are inside inserted fused cone.
Purity ≥95.0% (HPLC)
Storage Temp. room temp
UNSPSC 12171500

The following items have been added to your cart:

Choose a favorite list for this item:
Catalog Number Description Price
$

Returns/Order support

Please fill out the form below if you want to request order support from Krackeler Scientific.


Quick Order

* Required


New Tariffs & Effects on Pricing

Tariff note: As we navigate the current environment of tariff surcharges and mid-year manufacturer price increases, we want to keep you informed. While we have avoided making specific decisions thus far, we anticipate that we will be forced to implement a cost adjustment policy to offset the fees we have already been absorbing. We are committed to transparency and moderation in this process.

800-334-7725
office@krackeler.com


Play Video

To Request a Quote

  1. Search or Browse for items and add to them to your Shopping Cart.
  2. Click the "Request Quote" button at the bottom of the Shopping Cart page.
  3. Fill out required fields.
  4. Optionally you can convert to standard checkout mode by choosing a payment type.
  5. Click "Request Quote" at the bottom of the page.

You will be contacted with a quote.

To Order From a Quote

  1. Register and login to the website.
  2. Receive a quote from your sales representative or customer service.
  3. Have your copy of the quote in hand.
  4. Visit our quote module to search for your quote.
Back to Top