Eosin Methylene blue
SIGMA/32855 - according to May-Grünwald
MDL Number: MFCD00005038
Product Type: Chemical
| εmax | ≥1000 at 635-655 nm in methanol |
| ≥650 at 512-532 nm in methanol | |
| application(s) | diagnostic assay manufacturing hematology histology |
| form | powder or crystals |
| InChI | 1S/C21H10Br4O5.K/c1-29-21 |
| InChI key | NXBZXYJXNIFCRJ-UHFFFAOYSA |
| quality | according to May-Grünwald |
| Quality Level | 200  |
| SMILES string | [K+].COC(=O)c1ccccc1C2=C3 |
| storage temp. | room temp |
| Application: | Eosin methylene blue was used for staining cells, during optical microscopy studies, done for analysis of cell morphology. |
| General description: | Eosin has been used in combination with methylene blue, to detect carboxlic acid group in organic compounds. It gives a red or orange-coloured precipitate on treatment with carboxylic acid solution in water, acetone, alcohol, or dioxin. It is also used as an indicator for differentiation of the colon from the typhoid-dysentery group of bacteria. |
| General description: | Mixture of eosin and methylene blue stains for use in differential microbiological media to culture and identify pathogenic microorganisms, e.g., Neisseria sicca,, Acinetobacter calcoaceticus. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H319 |
| Precautionary statements | P280 - P305 + P351 + P338 - P337 + P313 |
| Hazard Codes | Xn |
| Risk Statements | 22-41 |
| Safety Statements | 26-39 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | room temp |
| Colour Index Number | 881/1038; 45380/52015 |
| UNSPSC | 12171500 |