Human Aortic Smooth Muscle Cells: HAOSMC, adult
SIGMA/354-05A
Synonym: HAOSMC cells
Product Type: Product-on-demand
Cell Applications : (A) Human Aortic Smooth Muscle Cells, HAOSMC. HAOSMC immunolabeled for smooth muscle-specific alpha-actin by ABC method (B, red) and by immunofluorescence (C, green). Nuclei are visualized with DAPI (C, blue).
| biological source | human aorta (normal, tunica intima and tunica media, fibrous plaque-free) |
| growth mode | Adherent |
| karyotype | 2n = 46 |
| manufacturer/tradename | Cell Applications, Inc |
| morphology | smooth muscle |
| packaging | pkg of 500,000 cells |
| Quality Level | 100  |
| relevant disease(s) | cardiovascular diseases |
| shipped in | dry ice |
| storage temp. | −196°C |
| technique(s) | cell culture | mammalian: suitable |
| Application: | aortic wall contraction, role of smc under normal conditions, blood pressure, distribution of oxygenated blood through systemic circulation |
| Cell Line Origin: | Aorta |
| Disclaimer: | RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization. |
| General description: | Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details. Human Aortic Smooth Muscle Cells (HAOSMC) provide an excellent model system to study all aspects of cardiovascular function and disease, especially those related to mechanisms of hyperplasia and hypertrophy of intimal smooth muscle cells leading to vascular occlusion in atherosclerosis and stent restenosis. HAOSMC have been utilized in dozens of research studies, for example, to:elucidate cytokines and growth factors signaling pathways implicated in the molecular regulation of smooth muscle cell proliferation, migration, and overall vascular function (Liu, 2009; Tan, 2009; Seymour, 2010; Chen, 2011; Hirase, 2013; Stein, 2013): • Understand hyperglycemia-related risk factors for atherosclerosis in diabetes patients (Maier, 2010), as well as effects of ethanol on vascular calcification (Oros, 2012) • Investigate extracellular elastic matrix deposition and its role in cardiovascular health, repair of damaged vasculature and successful tissue engineering (Gacchina, 2010, 2011; Venkataraman, 2012; Phillippi, 2013) • Study mechanisms and effects of mechanoregulation on proliferation and function of smooth muscle cells (Jiang, 2009; Venkataraman, 2013, 2014), and develop advanced stent technology, including novel surface materials, and drug and gene delivery systems to prevent restenosis and other occlusive vasculopathies (Brito, 2010; Deshpande, 2013; Kakade, 2013; Lamichhane, 2013) Characterization: Positive for smooth muscle cell specific alpha-actin expression. |
| Preparation Note: | • Can be cultured at least 16 doublings |
| Subculture Routine: | Please refer to the HAOSMC Culture Protocol . |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −196°C |
| UNSPSC | 41106514 |