DCFHDA
SIGMA/35845 - BioReagent, suitable for fluorescence, ≥95% (HPLC)
Synonym: 2′,7′-Dichlorofluorescein diacetate; Diacetyldichlorofluorescein
CAS Number: 2044-85-1
Empirical Formula (Hill Notation): C24H14Cl2O7
Molecular Weight: 485.27
MDL Number: MFCD00037501
Linear Formula: C24H14Cl2O7
Product Type: Chemical
| assay | ≥95% (HPLC) |
| fluorescence | λex 504 nm; λem 524 nm in 0.1 M Tris pH 8.0 (esterase) |
| InChI | 1S/C24H14Cl2O7/c1-11(27)3 |
| InChI key | VQVUBYASAICPFU-UHFFFAOYSA |
| mp | 232-234 °C (lit.) |
| product line | BioReagent |
| Quality Level | 100  |
| SMILES string | CC(=O)Oc1cc2Oc3cc(OC(C)=O |
| solubility | DMSO: soluble |
| storage temp. | 2-8°C |
| suitability | suitable for fluorescence |
| Application: | 2′,7′-Dichlorofluorescein diacetate is predominantly used to detect reactive oxygen (ROS) intermediates and quantify the overall oxidative stress in cells. Dcfh-DA is used in the quantitative assay to measure oxidative stress in nanoparticle-treated cells. |
| General description: | 2′,7′-Dichlorofluorescein diacetate, also known as DCFHDA or Dcfh-DA, is a sensitive cell-permeable redox probe. The lipophilic non-fluorescent Dcfh-DA readily crosses the cell membrane through passive diffusion followed by deacetylation. The deacylated product is an oxidant sensitive 2′,7′-dichlorofluorescein (DCHF). DCHF is oxidized later to form highly fluorescent DCF, which is measured. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥95% (HPLC) |
| mp | 232-234 °C (lit.) |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352108 |