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2′,7′-Dichlorofluorescein

SIGMA/35848 - BioReagent, suitable for fluorescence, ≥90% (T)

Synonym: DCF; Dichlorofluorescein

CAS Number: 76-54-0
Empirical Formula (Hill Notation): C20H10Cl2O5
Molecular Weight: 401.20
EC Number: 200-968-6
MDL Number: MFCD00005047
Linear Formula: C20H10Cl2O5
Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-35848-5G 5 g
 $96.40
1/EA		 Add To Favorites
45-35848-25G 25 g
 $491.00
1/EA		 Add To Favorites

 

assay ≥90% (T)
fluorescence λex 504 nm; λem 529 nm in 0.1 M Tris pH 8.0
form solid
InChI 1S/C20H10Cl2O5/c21-13-5-11-17(7-15(13)23)26-18-8-16(24)14(22)6-12(18)20(11)10-4-2-1-3-9(10)19(25)27-20/h1-8,23-24H
InChI key VFNKZQNIXUFLBC-UHFFFAOYSA-N
mp 280 °C (dec.) (lit.)
product line BioReagent
Quality Level 100 
SMILES string Oc1cc2Oc3cc(O)c(Cl)cc3C4(OC(=O)c5ccccc45)c2cc1Cl
suitability suitable for fluorescence
Application: 2′,7′-Dichlorofluorescein is used as fluorescent probe to measure ROS (Reactive Oxygen Species) for biological studies. It may also be used as a dip reagent for the fluorometric determination of vicinal diols, glycosides, and phenols.
General description: 2′,7′-Dichlorofluorescein, also known as DCF is a fluorescent dye. The DCF fluorescence excitation is at 498nm with emission at 522nm. The lipophilic non-fluorescent Dcfh-DA readily crosses the cell membrane through passive diffusion followed by deacetylation. The deacylated product is an oxidant sensitive 2′,7′-dichlorofluorescein (DCHF). DCHF is oxidized later to form highly fluorescent DCF, which is measured.
Hazard Codes Xi
Risk Statements 36/37/38
Safety Statements 26-36
RIDADR NONH for all modes of transport
WGK Germany WGK 3
Flash Point(F) Not applicable
Flash Point(C) Not applicable
Purity ≥90% (T)
mp 280 °C (dec.) (lit.)
UNSPSC 12352200

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