KBA.62 (KBA.62) Mouse Monoclonal Antibody
SIGMA/366M-9
Product Type: Chemical
Immunohistochemistry KBA.62 (KBA.62) on melanoma tissue
| antibody form | culture supernatant |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | KBA.62, monoclonal |
| conjugate | unconjugated |
| control | melanoma |
| description | For In Vitro Diagnostic Use in Select Regions (See Chart) |
| form | buffered aqueous solution |
| isotype | IgG1 |
| manufacturer/tradename | Cell Marque® |
| packaging | bottle of 1.0 mL predilute (366M-97) |
| bottle of 7.0 mL predilute (366M-98) | |
| vial of 0.1 mL concentrate (366M-94) | |
| vial of 0.5 mL concentrate (366M-95) | |
| vial of 1.0 mL concentrate (366M-96) | |
| Quality Level | 100  |
| 500 |
|
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | 2-8°C |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:25-1:100 |
| visualization | membranous |
| General description: | Anti-KBA.62 (Melanoma Associated Antigen) is a novel anti-melanoma antibody. Studies thus far have shown a similar sensitivity to melanocytic proliferations as that seen with S-100 protein staining, which is somewhat higher than that seen with anti-HMB-45. This has been confirmed by one study on a series of 215 sentinel lymph nodes. Moreover, anti-KBA.62 identified 6 patients (3%) who had confirmed sentinel lymph node metastasis but stained negative using anti-HMB-45. In this setting, the resolution appears to be higher than S-100 protein in that the staining pattern (membranous) is quite distinct. Interestingly, most cases of desmoplastic and spindle cell melanomas show strongly positive results with anti-KBA.62, unlike that seen with other melanocyte markers. It should be noted that anti-KBA.62 will label occasional endothelial cells which can serve as an internal positive control. A small percentage of well-differentiated squamous cell carcinomas of the skin (and lung) have also been noted to stain with this antibody; however, the poorly-differentiated forms of carcinoma do not, thus resolving a greater practical problem in differential diagnosis. Anti-KBA.62 is a useful additional marker for melanoma, specifically in desmoplastic/spindle cell cases and in the context of micrometastasis in sentinel lymph node. |
| Legal Information: | Cell Marque is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Other Notes: | For Technical Service please contact: 800-665-7284 or email: service@cellmarque.com |
| Other Notes: | KBA.62 Positive Control Slides, Product No. 366S , are available for immunohistochemistry (formalin-fixed, paraffin-embedded sections). |
| Physical form: | Solution in Tris Buffer, pH 7.3-7.7, with 1% BSA and <0.1% Sodium Azide |
| Preparation Note: | Download the IFU specific to your product lot and format  Note: This requires a keycode which can be found on your packaging or product label. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |