Quantification kit for oxidized and reduced glutathione
SIGMA/38185 - 200 tests
Synonym: γ-L-glutamyl-L-cysteinylglycine kit; Quantification kit
Product Type: Chemical
quality | 200 tests |
Quality Level | 100 |
storage temp. | 2-8°C |
Analysis Note: | Glutathione Quantification Kit contains a GSH masking reagent. This enables GSSG detection in the sample by measuring the absorption (λmax = 412 nm) obtained from a colorimetric reaction of DTNB (5,5’-dithiobis (2-nitrobenzoic acid)) coupled with the enzymatic recycling system. The total quantity of GSH can be measured by subtracting the amount of GSSG from the total amount of glutathione. |
Application: | Glutathione Quantification Kit is used to detect the total amount of glutathione as an index for oxidative stress or disease risk. |
Components: | Glutathione Quantification Kit contains: • Enzyme Solution (50µl X 1) • Buffer Solution (60ml X 1) • Standard GSH (X 1) • Masking Reagent (20µl X 1) • Co-enzyme (X 2) • Substrate (DTNB) (X4) • Standard GSSG (X 1) |
Features and Benefits: | The detection ranges of total glutathione and GSSG using in this kit are from 0.5 μmol/l to 50 μmol/l and from 0.5 μmol/l to 25 μmol/l, respectively. |
General description: | Glutathione Quantification Kit is designed to detect oxidized and reduced Glutathione. Glutathione is usually represented in a reduced form called GSH; however, GSH is converted into its oxidized state (GSSG) upon oxidative stress. The ratio between GSH and GSSG is considered an index for oxidative stress. Glutathione, also known as γ-L-glutamyl-L-cysteinylg |
Other Notes: | not available in Korea, Singapore, Taiwan and Japan |
Symbol | GHS02,GHS07 |
Signal word | Danger |
Hazard statements | H225 - H315 - H317 - H319 |
Precautionary statements | P210 - P233 - P240 - P280 - P303 + P361 + P353 - P305 + P351 + P338 |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 3 |
Storage Temp. | 2-8°C |
UNSPSC | 12352200 |