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Quantification kit for oxidized and reduced glutathione

SIGMA/38185 - 200 tests

Synonym: γ-L-glutamyl-L-cysteinylglycine kit; Quantification kit

Product Type: Chemical

Catalog Number PKG Qty. Price Quantity
45-38185-1KT 1 kit
$1170.00
1/EA
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quality 200 tests
Quality Level 100 
storage temp. 2-8°C
Analysis Note: Glutathione Quantification Kit contains a GSH masking reagent. This enables GSSG detection in the sample by measuring the absorption (λmax = 412 nm) obtained from a colorimetric reaction of DTNB (5,5’-dithiobis (2-nitrobenzoic acid)) coupled with the enzymatic recycling system. The total quantity of GSH can be measured by subtracting the amount of GSSG from the total amount of glutathione.
Application: Glutathione Quantification Kit is used to detect the total amount of glutathione as an index for oxidative stress or disease risk.
Components: Glutathione Quantification Kit contains: •  Enzyme Solution (50µl X 1)
• Buffer Solution (60ml X 1)
• Standard GSH (X 1)
• Masking Reagent (20µl X 1)
• Co-enzyme (X 2)
• Substrate (DTNB) (X4)
• Standard GSSG (X 1)
Features and Benefits: The detection ranges of total glutathione and GSSG using in this kit are from 0.5 μmol/l to 50 μmol/l and from 0.5 μmol/l to 25 μmol/l, respectively.
General description: Glutathione Quantification Kit is designed to detect oxidized and reduced Glutathione. Glutathione is usually represented in a reduced form called GSH; however, GSH is converted into its oxidized state (GSSG) upon oxidative stress. The ratio between GSH and GSSG is considered an index for oxidative stress. Glutathione, also known as γ-L-glutamyl-L-cysteinylglycine, is a tripeptide compound that is involved in anti-oxidation and drug metabolism. It acts as a substrate for enzymes like glutathione peroxidase, glutathione S-transferase, and thiol transferase.
Other Notes: not available in Korea, Singapore, Taiwan and Japan
Storage Temp. 2-8°C
UNSPSC 12352200

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