Abberior® STAR 580, NHS ester
SIGMA/38377 - for STED application
Product Type: Chemical
| assay | ≥70.0% (degree of coupling) |
| fluorescence | λex 583 nm; λem 605.0 nm±10 nm in PBS, pH 7 |
| Quality Level | 100  |
| solubility | DMF: 1 mg/mL, clear |
| storage temp. | −20°C |
| Application: | Anti-Mouse IgG-Abberior® STAR 580 antibody produced in goat has been used for STED (stimulated emission depletion) microscopy in CA3 (Cornus Ammonis) sections. Abberior® STAR 580 conjugated with secondary antibody has been used for dual-color STED imaging of cultured hippocampal neurons. |
| General description: | Abberior STAR 580 is the latest development for STED microscopy with a fluorescent dye in the orange regime. The dye can be excited from 550 to 580 nm. Abberior STAR 580 can substitute dyes like Atto™ 590/594 or AlexaFluor 584/594. The dye can most effectively be depleted in STED microscopy at 700 to 780 nm via e.g. a Ti:Sa laser or IR diodes. Abberior STAR 580 is the dye of choice for orange fluorescence. Moreover, the dye is particularly designed and tested for 2-color STED microscopy in combination with Abberior STAR 635 using 2 separate STED wavelength. This offers the great advantage of using 2 regular (not featuring a long Stokes-shift) dyes, which are in general more photostable. Please see our 2-color dye section Key features • Exceptionally bright orange fluorescent dye • Ideal for STED microscopy at 700-775 nm • 2-color labeling partner with STAR 635P for 2-color STED microscopy Absorption Maximum, λmax:: 587 nm (PBS, pH 7.4; water) 584 nm (MeOH, aq. ACN) Extinction Coefficient, ε(λmax): 85,000 M-1cm-1 (PBS, pH 7.4) Correction Factor, CF260 = ε260/εmax: 0.17 (PBS, pH 7.4; water) Correction Factor, CF280 = ε280/εmax: 0.15 (MeOH, aq. ACN) Fluorescence Maximum, λfl: 607 nm (PBS, pH 7.4) 604 nm (MeOH, aq. ACN) Recommended STED Wavelength, λSTED: 700 - 780 nm Fluorescence Quantum Yield, η: 0.90 (PBS, pH 7.4) Fluorescence Lifetime, τ: 3.5 ns (PBS, pH 7.4) |
| Legal Information: | abberior is a registered trademark of Abberior GmbH |
| Legal Information: | Atto is a trademark of Atto-Tec GmbH |
| Other Notes: | Find more infomation here Abberior® |
| Suitability: | Designed and tested for fluorescent super-resolution microscopy |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥70.0% (degree of coupling) |
| Storage Temp. | −20°C |
| UNSPSC | 12352108 |