Leukocyte Peroxidase (Myeloperoxidase) Kit
SIGMA/391A - DAB substrate kit
MDL Number: MFCD00131641
Product Type: Chemical
Staining image is the result of Procedure No. 391.
| application(s) | hematology histology |
| IVD | for in vitro diagnostic use |
| Quality Level | 500  |
| shelf life | Expiry date on the label. |
| shipped in | wet ice |
| storage temp. | 2-8°C |
| usage | sufficient for 10-120 manual assays |
| Application: | Diaminobenzidine, as a benzidine substitute for myeloperoxidase, has less tinctorial power than benzidine. This made DAB much less attractive to histochemists. Hanker and associates improved DAB methodology. According to their modification, the brown reaction product is first intensified with copper salts and followed by application of Gill′s modified Papanicolaou stain, resulting in intense grey-black staining at sites of neutrophil and monocyte peroxidase. |
| Application: | The Leukocyte Peroxidase (Myeloperoxidase) kit is intended for use as a general laboratory histochemical stain for the demonstration of leukocyte peroxidase. This manual, qualitative procedure identifies leukocyte peroxidase in blood or bone marrow films of human specimens. |
| General description: | Used to demonstrate polymorphonuclear cells in blood or bone marrow films. Each kit has sufficient reagents for 10 uses. |
| Symbol |       GHS02,GHS03,GHS05,GHS07,GHS08,GHS09 |
| Signal word | Danger |
| Hazard statements | H225 - H272 - H290 - H302 + H312 + H332 - H315 - H317 - H318 - H334 - H335 - H341 - H350 - H360FD - H370 - H373 - H41 |
| Precautionary statements | P201 - P210 - P220 - P260 - P273 - P280 - P304 + P340 + P312 - P305 + P351 + P338 + P310 - P308 + P311 - P370 |
| Hazard Codes | O,F,T,N |
| Risk Statements | 45-8-11-23/24/25-37/38-39/23/24/25-41-42/43-50/53-68 |
| Safety Statements | 53-23-26-36/37/39-45-61 |
| RIDADR | UN 3316 9 |
| Flash Point(F) | 57.2 °F |
| Flash Point(C) | 14 °C |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352200 |
| Components | Diaminobenzidine 10 vial(s); Copper nitrate 2 vial(s); Gill Modified EA solution 100 mL; Glutaraldehyde solution 2 x 75 mL; Hematoxylin Solution, Gill No. 3 100 mL; Scott's Tap Water Substitute Concentrate 100 mL; TRIZMAL™ Buffer Concentrate 50 mL |