Human Chondrocytes - Osteoarthritis: HC-OA, adult
SIGMA/402OA-05A
Synonym: HC-OA cells
Product Type: Product-on-demand
Cell Applications : Human Chondrocytes-Osteoarthritis, HC-OA
| biological source | human articular cartilage (osteoarthritic) |
| growth mode | Adherent |
| karyotype | 2n = 46 |
| manufacturer/tradename | Cell Applications, Inc |
| morphology | chondrocyte |
| packaging | pkg of 500,000 cells |
| Quality Level | 100  |
| relevant disease(s) | arthritis |
| shipped in | dry ice |
| storage temp. | −196°C |
| technique(s) | cell culture | mammalian: suitable |
| Application: | model for studying chondrocyte biology in an osteoarthritis joint |
| Cell Line Origin: | Cartilage |
| Components: | Basal Medium containing 10% FBS & 10% DMSO |
| Disclaimer: | RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization. |
| General description: | Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details. HC-OA Human Chondrocytes are derived from human articular cartilage of donors with osteoarthritis (OA). Osteoarthritis is an inflammatory disease characterized by increased degradation of cartilage tissue in the joint due to overproduction of enzymes degrading the extracellular matrix. Despite the initial proliferation and activation of chondrocytes, they are not able to efficiently repair the degrading cartilage. Instead, chondrocytes undergo terminal differentiation and eventually apoptose, leading to mineralization of cartilage in a process resembling bone formation during development. Thus, HC-OA provide a useful model to study changes in chondrocyte biology in response to abnormal environment of the OA joint. Characterization: Positive for aggrecan after differentiation. HC-OA have been used to demonstrate that THF-alpha activates transcription of ADAMTS-4, a metalloproteinase implicated in cartilage degradation in OA, via p38-MAPK-dependent mechanism (Xue, 2013). |
| Preparation Note: | • 1st passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO • Can be cultured at least 10 doublings |
| Subculture Routine: | Please refer to the HC-OA Culture Protocol . |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −196°C |
| UNSPSC | 41106514 |